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Supplementation of Eriocitrin in Intestinal Microbiota

Not Applicable
Terminated
Conditions
Pre Diabetes
Interventions
Dietary Supplement: Eriocitrin
Registration Number
NCT03925909
Lead Sponsor
São Paulo State University
Brief Summary

Pre-diabetes is characterized by high plasma concentration of glucose and glycated hemoglobin and is the main risk factor for the development of type 2 diabetes. Several studies show that the intestinal microbiota is intimately linked to cardio-metabolic factors (type 2 diabetes, insulin resistance) when in situations of dysbiosis. Food is a key element for a healthy microbiota, focusing on the consumption of polyphenols that modulate the intestinal environment through its alteration and production of short chain fatty acids, and can thus be a way of reversing situations such as pre- diabetes and insulin resistance. The objective of the study will be to investigate whether chronic supplementation of eriocitrin alters the intestinal microbiota of pre-diabetic and insulin resistant individuals, reversing these situations. This will be done by supplementation of eriocitrin-containing capsules with different dosages in pre-diabetic and insulin resistant individuals. There will be 12 weeks of intervention, with faecal collections, anthropometric and dietary evaluation, and then will be made microbiological analysis to identify the intestinal microbiota and biochemical analysis before and after the intervention. For statistical analysis, normality and homogeneity test (Kolmogorov-Smirnov and Levine test respectively), T-test to compare baseline time between groups and repeated-measures ANOVA (two-way) were used to compare changes within and between groups.

Detailed Description

Individuals of both sexes between 30 and 60 years of age who present fasting glycemia between 100 mg / dL (5,6 mmol / L) and 125 mg / dL (6,9 mmol / L) 50, or concentration of glycated hemoglobin between 5.7% (39 mmol / mole) and 6.4% (47 mmol / mole) 50, or glycemic concentrations in the oral glucose tolerance test between 140mg / dL (7.8 mmol / L) to 199mg / dL (11.0 mmol / L) 50. The study will also include insulin-resistant individuals: insulin above 25 uU / mL, or who present HOMA-IR score above 2.7.

Individuals using supplements (vitamins, bioflavonoids), pregnant women and those who practice intense physical exercise (\> 10h / week) will be excluded from the study.

The primary endpoint will be alteration of the microbiota with possible improvement of the glycemic profile, leading to the reversal of pre-diabetes and insulin resistance.

Recruitment & Eligibility

Status
TERMINATED
Sex
All
Target Recruitment
40
Inclusion Criteria
  • 30-60 years
  • Increased fasting blood glucose from 6.1 to 7.0 mmol / L or,
  • Decreased glucose tolerance of 7 , 8 to 11.1 mmol / L or,
  • Glycated hemoglobin with values between 5.7 and 6.4%
Exclusion Criteria
  • Use hypoglycemic, hypolipidemic drugs,
  • Use dietary supplements (vitamins, minerals, bioflavonoids, prebiotics, probiotics or other bioactive compounds),
  • Exercise intensely (more than 10 hours per week)
  • History of cardiovascular diseases , diabetes mellitus, liver and kidney disease.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Eriomin 400 mgEriocitrinThe volunteers will receive one capsule containing 400 mg eriocitrin
Eriomin 200 mgEriocitrinThe volunteers will receive one capsule containing 200 mg eriocitrin
Eriomin 800 mgEriocitrinThe volunteers will receive one capsule containing 800 mg eriocitrin
Primary Outcome Measures
NameTimeMethod
Rate of change in microbiome0-12 week

Changes in microbiome before and after administration of the intervention / placebo

Secondary Outcome Measures
NameTimeMethod
Rate of change in plasma glucose concentration0-12 week

Changes in serum 2 hours after oral glucose tolerance test, HbA1c, insulin, HOMA-IR before and after administration of the intervention / placebo

Trial Locations

Locations (1)

Sao Paulo State University "Julio de Mesquita Filho"

🇧🇷

Araraquara, SP, Brazil

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