Monocyte Subsets Altered by Anesthesia

Completed

• Conditions: Regional Anesthesia; Immune Function; General Anesthesia

• Registration Number: NCT03431532
• Lead Sponsor: University Hospital, Saarland

Brief Summary

The impact of different anesthetic techniques on the immune system remains unclear.

Aim of this ex vivo / in vitro study was to determine the effects of general and neuraxial anesthesia on monocyte subset alteration and the release of prototypical pro- and anti-inflammatory cytokines. Twenty patients undergoing total knee replacement surgery were randomly assigned to receive either general anesthesia (ITN) or combined spinal/epidural anesthesia (CSE). CD14 and HLA-DR expression patterns on monocytes and intracellular TNF-alpha production were quantified via flow cytometry. TNF-α and IL-10 release were measured via enzyme linked immunosorbent assay (ELISA).

Detailed Description

Numerous of factors affect the immunological response during surgery. Despite intensive research, the impact of different anesthetic techniques on the immune system remains unclear.

Aim of this ex vivo / in vitro study was to determine the effects of general and neuraxial anesthesia on monocyte subset alteration and the release of prototypical pro- and anti-inflammatory cytokines. Twenty patients undergoing total knee replacement surgery were randomly assigned to receive either general anesthesia (ITN) or combined spinal/epidural anesthesia (CSE). Samples of venous blood were taken from the patients before and after induction of anesthesia, immediately, 6 hours, 24 hours, and 48 hours after surgery. All blood samples were incubated in presence or absence of LPS (lipopolysaccharide; 1 µg/ml) for 24 hours. CD14 and HLA-DR expression patterns on monocytes and intracellular TNF-alpha production were quantified via flow cytometry. TNF-α and IL-10 release were measured via enzyme linked immunosorbent assay (ELISA).

Study Timeline

• Study Start: 2013-02-01
• Primary Completion: 2015-01-01
• Study Completion: 2015-03-01
• Study First Submitted: 2018-01-30
• Status Verified: 2018-02
• Study First Submitted QC: 2018-02-11
• Study First Posted: 2018-02-13
• Last Update Submitted: 2022-05-10
• Last Update Posted: 2022-05-11

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 20

Inclusion Criteria

• Total knee replacement surgery
• 18 years or older

Exclusion Criteria

• clinical signs of infection,
• neoplasia
• treatment with immunomodulatory agents
• any kind of immune mediated disease.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
CD 14 and HLA-DR Expression Patterns on monocytes	1 year	CD 14 and HLA-DR Expression Patterns on monocytes in whole blood culture assay

Secondary Outcome Measures

Name	Time	Method
Intracellular TNF-alpha production	1 year	Intracellular TNF-alpha production in whole blood culture assay
TNF-alpha and IL-10 release	1 year	TNF-alpha and IL-10 release in whole blood culture assay

Trial Locations

Locations (1)

Sven O. Schneider, MD; 🇩🇪 Homburg, Saarland, Germany