The Effect of Exercise and Presleep Nutrition on Muscle Connective Tissue Remodelling
- Conditions
- Interventional
- Interventions
- Dietary Supplement: Collagen and Vitamin CDietary Supplement: Carbohydrate Placebo
- Registration Number
- NCT06054620
- Lead Sponsor
- University of Toronto
- Brief Summary
The primary objective of this study will be to determine the influence of a presleep vitamin C-enriched, collagen-modelled supplement (CVC) on the metabolic fate of dietary glycine using a \[2H5\]glycine tracer within the intramuscular connective tissue at rest and after a bout of resistance exercise. Other outcomes will be related to regulation collagen remodelling in skeletal muscle.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- RECRUITING
- Sex
- All
- Target Recruitment
- 12
- Healthy, recreationally active, male and female participants.
- Healthy will be defined as someone who does not have a health condition that would compromise their ability to safely participate in the strenuous physical activity involved in our study as screened for by the Get Active Questionnaire.
- Willing to abstain from resistance exercise for 2 weeks prior to the first metabolic trial and for the duration of the study.
- Participants must be able to abstain from lower-body resistance and/or plyometric exercise for at least 2 weeks prior to the first metabolic trial.
- Participants will be aged 18-35 years old.
- Participants must be normal to overweight (e.g., BMI 18.5-30).
- Participants are willing to abide by the compliance rules of this study (e.g., abstain from physical activity and alcohol 48h prior to each trial).
- Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants).
- Inability to adhere to any of the compliance rules judged by principal investigator or medical doctor.
- Self-reported regular tobacco use.
- Self-reported illicit drug use (e.g. growth hormone, testosterone, etc.).
- Regular use of anti-inflammatory drugs (e.g., ibuprofen).
- Self-reported regularly sleeping less than an average of 7 hours over 3 days (minimum recommended daily amount).
- Individuals who have participated in studies within the past year involving any of the stable isotopes in the study.
- Use of birth control and discontinued use in the last 3-months (female only).
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description Collagen and Vitamin C Collagen and Vitamin C - Carbohydrate Placebo Carbohydrate Placebo -
- Primary Outcome Measures
Name Time Method Enrichment of Muscle Collagen 9 hours Overnight incorporation of orally ingested amino acid tracer (D5 Gly and D5 Phe) into mature collagen protein isolated from skeletal muscle biopsy samples taken 9 hours after consumption of the tracer-containing supplement (CVC or PLA) and rest or resistance exercise. Enrichment will be quantified via tandem LC-MS/MS.
- Secondary Outcome Measures
Name Time Method Area of muscle with localized collagen remodelling 9 hours Immunofluorescence determined localization of collagen breakdown and synthesis using collagen hybridizing peptide and procollagen antibodies, respectively, on human skeletal muscle cross-sections.
Concentration of Urinary Growth Hormone 8 hours Urine will be collected overnight to measure growth hormone secretion using chemiluminescence.
Enrichment of Myofibrillar Protein 9 hours Overnight incorporation of orally ingested amino acid tracer (D5 Gly and D5 Phe) into myofibrillar proteins isolated from skeletal muscle biopsy samples taken 9 hours after consumption of the tracer-containing supplement (CVC or PLA) and rest or resistance exercise. Enrichment will be quantified via tandem LC-MS/MS.
Trial Locations
- Locations (1)
Goldring Centre for High Performance Sport at the University of Toronto
🇨🇦Toronto, Ontario, Canada