Incidence, Risk Factors and Microbiological Features of Infective Keratitis in Cairo University Hospitals
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Infective Keratitis
- Sponsor
- Kasr El Aini Hospital
- Enrollment
- 85
- Locations
- 1
- Primary Endpoint
- To detect incidence of infective keratitis amongst all ophthalmological casualty cases by documenting the number of cases presenting or referred to the casualty of the ophthalmology department.
- Status
- Completed
- Last Updated
- 3 years ago
Overview
Brief Summary
The aim of the study was to identify the incidence of infective keratitis presenting to the ophthalmology emergency department at Cairo University hospital, the risk factors and the bacterial/fungal spectrum causing the ulcers and to determine the best possible empirical therapy followed by specific therapy after obtaining culture results.
Investigators
Nevien Lotfy Abdelkader
Ophthalmology Resident at the Ophthalmology Department, Cairo University.
Kasr El Aini Hospital
Eligibility Criteria
Inclusion Criteria
- •Patients presenting at the casualty who are clinically diagnosed as infectious keratitis , of all age groups and including both genders.
Exclusion Criteria
- •Patients presenting with clinical picture suggestive of
- •Viral keratitis solely without superimposed secondary infection.
- •Non infectious keratitis as peripheral ulcerative keratitis ( e.g. Mooren's ulcer, marginal keratitis, autoimmune keratitis )
Outcomes
Primary Outcomes
To detect incidence of infective keratitis amongst all ophthalmological casualty cases by documenting the number of cases presenting or referred to the casualty of the ophthalmology department.
Time Frame: through study completion, an average of eight months
Noting down the number of patients diagnosed with microbial keratitis that present to the casualty of a tertiary referral hospital, and calculate the percentage amongst all other ophthalmic casualty cases that present to the emergency as well.
To identify risk factors and the most common causative organisms of microbial keratitis among the Egyptian population through history taking from each patient and the results of corneal sampling taken from the ulcer.
Time Frame: Three weeks for each participant
Thorough history taking including risk factors that contributed to the ulcer occurrence. Corneal sampling through corneal scraping from the base and edge of the ulcer that undergoes direct examination and culturing using standard laboratory protocols.
To detect antimicrobial susceptibility and resistance of different antimicrobial agents causing corneal infections in our study group.
Time Frame: two weeks for each participant
Antibiotic susceptibility testing was performed by the disc diffusion method (Modified Kirby Bauer technique) using Muller Hinton agar, aerobic incubation at 35°C for 16-18 hours. Anti fungal susceptibility was done using microdilution testing following the CLSI reference method.
Secondary Outcomes
- To assess the impact of culture and sensitivity results of corneal scrapings on the initial empirical therapy by calculating the number of cases that needed to shift the initial therapy to another one according to the antimicrobial susceptibility.(six months)
- Improving lines of management of microbial keratitis for a better outcome based on the cooperation between clinical ophthalmologists and microbiologists to reach a proper diagnosis and tailor the management accordingly.(six months)