Human Gut Microbiota and microRNA Expression

Not Applicable

• Conditions: microRNA; Gut Microbiota
• Interventions: Dietary Supplement: stachyose; Dietary Supplement: Maltodextrin

• Registration Number: NCT05392348
• Lead Sponsor: Xijing Hospital

Brief Summary

In this study, stachyose was used as an intervention factor. We will evaluate changes in fecal gut microbiota and miRNA expression profiles in subjects under stachyose intervention

Detailed Description

In the stachyose intervention group, each person in the stachyose intervention group took 5 g of stachyose daily before breakfast, and the administration method was 100 ml of drinking water dissolved and taken orally for two months. Each person in the placebo control group took the same amount of maltodextrin daily. Stool samples of the subjects were collected weekly.

Study Timeline

• Status Verified: 2021-06
• Study First Submitted: 2021-07-07
• Study First Submitted QC: 2022-05-24
• Last Update Submitted: 2022-05-24
• Study Start: 2022-05-25
• Study First Posted: 2022-05-26
• Last Update Posted: 2022-05-26
• Primary Completion: 2022-06-25
• Study Completion: 2022-06-30

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 36

Inclusion Criteria

• healthy human

Exclusion Criteria

• people who need intake antibiotics，probiotics or its product

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Stachyose intervention group	stachyose	Subjects with stachyose as intervention，5g/kg·bw/day.
Normal Control group	Maltodextrin	Subjects with maltodextrin as intervention，5g/kg·bw/day.

Primary Outcome Measures

Name	Time	Method
structure of gut microbiota	up to 4 weeks	16S rDNA sequencing valuated the gut microbiota composition in human
expression of microRNA	up to 4 weeks	small rna sequencing valuated the microRNA expression in human

Trial Locations

Locations (1)

the First Affiliate Hospital of the Air Force Medical University; 🇨🇳 Xi'an, Shaanxi, China