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Effect of Gemigliptin on Metabolic Endotoxemia and Lipemia

Not Applicable
Completed
Conditions
Type 2 Diabetes Mellitus
Interventions
Drug: Placebo
Registration Number
NCT02186080
Lead Sponsor
Seoul National University Hospital
Brief Summary

This is an exploratory study on the metabolic endotoxemia associated with type 2 diabetes. The investigators will measure systemic endotoxin level and lipid level after ingestion of high fat diet to evaluate the effect of gemigliptin, a DPP-4 inhibitor, on metabolic endotoxemia and lipemia induced by high fat diet.

Detailed Description

Study subjects will be allocated to either gemigliptin or placebo. After 4 weeks of treatment and 2 weeks of wash out period, all subjects will be switched to the opposite arm.

The subjects will be tested oral fat tolerance test and adipose tissue needle biopsy(only for the subjects who agreed to participate donate adipose tissue) at 3 time points: the start of 1st treatment, end of 1st treatment and end of 2nd treatment.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
10
Inclusion Criteria
  • Age from 20 to 75
  • At least 6 months from the diagnosis of type 2 diabetes
  • Current diabetes treatment: life style modification and/or metformin and/or sulfornylurea
  • No change of the diabetes treatment (drug number and dossage) in recent 3 months
  • HbA1c 6.5% to 10%
Exclusion Criteria
  • Recent cardiovascular event in 6 months
  • Concurrent use of statin or fibrate or ezetimibe
  • Renal failure, chronic liver disease
  • Pregnancy or lactation
  • Use of other DPP-4 inhibitor or GLP-1 analogue in recent 3 months

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
GemigliptinGemigliptinGemigliptin 50mg qd added to subjects current diabetes treatment
PlaceboPlaceboPlacebo (identical in appearance to gemigliptin)
Primary Outcome Measures
NameTimeMethod
Difference between baseline and peak serum LPSserum LPS before high fat diet and 15 min, 30 min, 1 hr, 2 hr, 3 hr, 4 hr after high fat diet

The increment of serum LPS after ingestion of high fat diet

Secondary Outcome Measures
NameTimeMethod
Fasting serum LPS levelAfter 10 hrs of fasting

Compare fasting LPS level between treatment group

Incremental AUC of serum LPS during oral fat tolerance testserum LPS before high fat diet and 15 min, 30 min, 1 hr, 2 hr, 3 hr, 4 hr after high fat diet

Calculated incremental AUC of serum LPS

Difference between baseline and peak serum triglyceride levelserum TG before high fat diet and 15 min, 30 min, 1 hr, 2 hr, 3 hr, 4 hr after high fat diet

The increment of serum TG after ingestion of high fat diet

Incremental AUC of serum triglyceride level during oral fat tolerance testserum TG before high fat diet and 15 min, 30 min, 1 hr, 2 hr, 3 hr, 4 hr after high fat diet

Calculated incremental AUC of serum TG

Difference between baseline and peak serum apolipoprotein-B48serum apolipoprotein-B48 before high fat diet and 15 min, 30 min, 1 hr, 2 hr, 3 hr, 4 hr after high fat diet

The increment of serum apolipoprotein-B48 after ingestion of high fat diet

Incremental AUC of serum apolipoprotein-B48 during oral fat tolerance testserum apolipoprotein-B48 before high fat diet and 15 min, 30 min, 1 hr, 2 hr, 3 hr, 4 hr after high fat diet

Calculated incremental AUC of serum apolipoprotein-B48

Serum level of inflammatory markers (CRP, TNF-alpha, IL-6)After 10 hr of fasting

Compare serum level of inflammatory markers between treatment groups

mRNA expression level of inflammatory markers in the adipose tissueAfter high fat diet ingestion

Compare mRNA expression level of inflammatory markers

Trial Locations

Locations (1)

Seoul National University Hospital

🇰🇷

Seoul, Korea, Republic of

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