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Study to Evaluate the Pharmacodynamics and Efficacy of Leuprolide Tablets (Ovarest®) in Women With Endometriosis

Registration Number
NCT05096065
Lead Sponsor
Enteris BioPharma Inc.
Brief Summary

The pharmacodynamic endpoint of percentage of subjects with suppressed estradiol level (less than 20 pg/mL) on cycle day 29 is the primary endpoint of the study.

Detailed Description

Objectives of this study:

1. To determine efficacy and pharmacodynamic effects of various dosing regimens of Ovarest® (within the 60-mg - 120 mg daily dosing range) in women with endometriosis.

2. To determine a minimally effective daily dosing regimen of Ovarest® with pharmacodynamic effects at least comparable to the historical data for marketed Lupron Depot formulations and for GnRH antagonists indicated for the treatment of endometriosis.

3. To evaluate safety and tolerability of the long-term administration of leuprolide within the targeted daily dosing range in women with endometriosis.

The most important goal of this study is to provide adequate dose-response data for the suppression of estradiol (E2) levels below the menopausal threshold of 20 pg/mL. Results of this study in conjunction of Enteris proprietary PK data will support further development of Leuprolide Acetate Oral Tablet for the treatment of reproductive disorders, particularly endometriosis. Another reason for this trial is to support a comparative evaluation of the PD effects across the QD and BID regimens delivering the same overall dose of Ovarest

Recruitment & Eligibility

Status
UNKNOWN
Sex
Female
Target Recruitment
16
Inclusion Criteria

Not provided

Exclusion Criteria

Subjects to whom any of the following applies will be excluded from the study:

  1. Hypersensitivity to GnRH, GnRH agonist analogs, similar nonapeptides, or any of the excipients in LUPRON DEPOT. Note: This is a contraindication from the Lupron Depot label.
  2. Undiagnosed abnormal vaginal bleeding. Note: This is a contraindication from the Lupron Depot label.
  3. Known or suspected pregnancy, or subjects who are considering becoming pregnant prior to the conclusion of this study. Note: LUPRON DEPOT is contraindicated in women who are or may become pregnant while receiving the drug. LUPRON DEPOT may cause fetal harm when administered to a pregnant woman.... If this drug is used during pregnancy, or if the patient becomes pregnant while taking this drug, the patient should be apprised of the potential hazard to the fetus.
  4. Breastfeeding or within 2 months after stopping breastfeeding (relative to the screening visit). Note: Use of LUPRON DEPOT is contraindicated in women who are breastfeeding.
  5. Thrombophlebitis, thromboembolic disorders, cerebral apoplexy, or a past history of these conditions. Note: Per the LUPRON DEPOT label, a possible coadministration of norethindrone acetate is contraindicated in women with thrombophlebitis, thromboembolic disorders, cerebral apoplexy, or a past history of these conditions.
  6. Markedly impaired liver function or liver disease. Note: Per the LUPRON DEPOT label, a possible coadministration of norethindrone acetate is contraindicated in women with markedly impaired liver function or liver disease.
  7. Known or suspected carcinoma of the breast. Note: Per the LUPRON DEPOT label, a possible coadministration of norethindrone acetate is contraindicated in women with known or suspected carcinoma of the breast.
  8. Status postpartum or postabortion within a period of 2 months prior to the screening visit
  9. History of significant alcohol or drug abuse within one year prior to the screening visit
  10. Clinically significant vital sign abnormalities (systolic blood pressure lower than 90 or over 140 mm Hg, diastolic blood pressure lower than 50 or over 90 mm Hg, or heart rate less than 50 or over 100 bpm) at screening (following recheck after five minutes at rest)
  11. Any clinically significant history or presence of neurologic, endocrinologic, pulmonary, hematologic, immunologic, or metabolic disease
  12. History of severe respiratory depression or pulmonary insufficiency
  13. Diabetes mellitus requiring insulin
  14. History of headaches with focal neurological symptoms
  15. Uncontrolled thyroid disorder
  16. Sickle cell anemia
  17. Current or history of clinically significant depression in the last year
  18. Known disturbance of lipid metabolism
  19. Hepatic adenoma or carcinoma
  20. Known or suspected endometrial carcinoma or estrogen-dependent neoplasia
  21. Clinically significant history or presence of any gastrointestinal pathology (e.g., chronic diarrhea, inflammatory bowel diseases, history of cholecystectomy), unresolved gastrointestinal symptoms (e.g., diarrhea, vomiting) or kidney disease, or other conditions known to interfere with the absorption, distribution, metabolism, or excretion of the drug
  22. Difficulty in swallowing study medication
  23. Any food allergy, intolerance, restriction or special diet that, in the opinion of the Investigator, could contraindicate the subject's participation in this study
  24. Positive test for hepatitis B, hepatitis C, or human immunodeficiency virus (HIV) at screening
  25. Administration of any investigational drug and/or experimental device within 30 days prior to the screening visit
  26. Administration of any biologics within 30 days prior to the screening visit. Note: The Covid-19 vaccine is not included in this prohibition.
  27. Clinically significant finding on the ECG suggesting participation in the study could pose a risk to the subject
  28. A depot injection or an implant of any drug within 1 month prior to the screening visit
  29. Use of oral contraceptives or other sex steroid hormones within 1 month prior to the screening visit. Note: A 1-month drug holiday period is mandatory for potential subjects receiving GnRH agonists and GnRH antagonists.
  30. Any clinically significant physical or gynecological abnormality at the screening visit
  31. Any clinically significant abnormal laboratory test result at the screening visit
  32. Hemoglobin <11.5 g/dL and/or hematocrit <32%
  33. Use of over-the-counter products containing any substances which could have the propensity to impact either estradiol or gonadotropin level
  34. Donation of plasma within 7 days prior to dosing. Donation or loss of blood (excluding volume drawn at screening) of 50 mL to 499 mL of blood within 30 days, or more than 499 mL within 56 days prior to the first dosing
  35. History of seizures, epilepsy, cerebrovascular disorders, central nervous system anomalies or tumors
  36. Significant risk factors for decreased bone mineral content and/or bone mass, such as family history (in a first degree relative) of osteoporosis, personal history of chronic use of corticosteroids or anticonvulsants
  37. Participation in another drug research within 1 month prior to screening
  38. Deemed by the Investigator to have questionable ability to comply with the study protocol, including inadequate adherence to both dosing and use of the AiCure medication adherence monitoring platform during the Run-in Period or during the three Treatment Cycles
  39. Current use of any prescription medication known to cause delayed gastric emptying (e.g. glucagon-like peptide-1 receptor agonists)

Study & Design

Study Type
INTERVENTIONAL
Study Design
SEQUENTIAL
Arm && Interventions
GroupInterventionDescription
Treatment B: Leuprolide Oral Tablet, 80 mg QDLeuprolide Oral Tablet - 80 mg - QD - Treatment BLeuprolide Oral Tablet (Ovarest), 80 mg (2 x 40 mg tablets), administered once daily (QD) for up to 35 consecutive days with food-intake restrictions.
Treatment C: Leuprolide Oral Tablet, 60 mg QDLeuprolide Oral Tablet - 60 mg - QD - Treatment CLeuprolide Oral Tablet (Ovarest), 60 mg, administered once daily (QD) for up to 29 consecutive days with food-intake restrictions.
Treatment E:Leuprolide Oral Tablet (Ovarest), 40 mg BIDLeuprolide Oral Tablet - 40 mg - BID - Treatment ELeuprolide Oral Tablet (Ovarest), 40 mg, administered twice daily (BID), 12 hours apart for up to 29 consecutive days with food-intake restrictions.
Treatment A: Leuprolide Oral Tablet, 120 mg QDLeuprolide Oral Tablet - 120 mg - QD- Treatment ALeuprolide Oral Tablet (Ovarest), 120 mg (2 x 60 mg tablets), administered once daily (QD), for up to 35 consecutive days with food-intake restrictions.
Treatment D: Leuprolide Oral Tablet, 60 mg BIDLeuprolide Oral Tablet - 60 mg - BID - Treatment DLeuprolide Oral Tablet (Ovarest), 60 mg, administered twice daily (BID), 12 hours apart for up to 35 consecutive days with food-intake restrictions.
Primary Outcome Measures
NameTimeMethod
Adequacy of suppression of estradiol (E2) as assessed by the subject incidence of estradiol level below 20 pg/mLTreatment cycle: Day 29

The primary PD metric - suppression of E2 level (E2 \<20 pg/mL) - will be assessed at each on-treatment evaluation timepoint. E2 below 20 pg/mL at Day 29 is a primary endpoint for a given Treatment Cycle.

Secondary Outcome Measures
NameTimeMethod
Composite Pelvic Signs and Symptoms (CPSS) scoresTreatment day 29

CPSS scores (composite and for the individual items) and changes from the pre-dosing baseline will be summarized by treatment received. CPSSS values are derived from the Biberoglu and Behrman scale, with 5 components addressing dysmenorrhea (0-none and 3-severe), dyspareunia (0-none and 3-severe), non-menstrual pelvic pain (0-none and 3-severe), pelvic tenderness (0-none and 3-severe), and pelvic induration (0-none and 3-severe). In composite score (total symptom and sign severity score) 0 being none and 11-15 being very severe.

Suppression of ovulation (as evidenced by progesterone levels <3 ng/mL)Treatment cycle: Day 22 and 29

It will be evaluated with Treatment Cycle Day 22 and Treatment Cycle Day 29 (separately and combined) positioned as most important timepoints for this analyte.

(Pre-dose leuprolide level) Subject incidence of Leuprolide below detectable levelTreatment Cycle: Days 1, 8, 15, 22 and 29 days

The summaries will be provided for each PK evaluation timepoint and across the entire treatment cycle

Luteinizing hormone (LH) levelsTreatment days: 1,8, 15, 22 and 29; Post dosing day 14

Serum concentration measured immediately prior to dosing

Follicle Stimulating Hormone (FSH) levelsTreatment days: 1,8, 15, 22 and 29; Post dosing day 14

Serum concentration measured immediately prior to dosing

Number of vaginal (menstrual) bleeding days28 days of therapy and 14 days post study follow up

The onset of menstrual period is defined as at least three consecutive bleeding/spotting days during the 14-day postdosing period.

Trial Locations

Locations (4)

Physician Care Clinical Research, LLC

🇺🇸

Sarasota, Florida, United States

Tidewater Clinical Research

🇺🇸

Norfolk, Virginia, United States

Complete Healthcare for Women

🇺🇸

Columbus, Ohio, United States

Seattle Clinical Research Center

🇺🇸

Seattle, Washington, United States

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