Modeling of Amyotrophic Lateral Sclerosis Using Patient Fibroblasts to Study Different Form of the Disease.
Overview
- Phase
- Not Applicable
- Intervention
- biopsy
- Conditions
- Amyotrophic Lateral Sclerosis
- Sponsor
- Assistance Publique - Hôpitaux de Paris
- Enrollment
- 110
- Locations
- 2
- Primary Endpoint
- Detection of cytoplasmic TDP-43, TIA1 and/or p62 aggregates in patient fibroblasts
- Status
- Recruiting
- Last Updated
- 19 days ago
Overview
Brief Summary
Amyotrophic Lateral Sclerosis (ALS) is the most common motor neuron disease in adults. This longitudinal study involves three cohorts of participants: patients with sporadic or hereditary ALS, asymptomatic individuals carrying pathogenic mutations responsible for ALS, and control subjects. In this study, a skin biopsy and blood sampling will be performed at the initial visit (M0), then at M12 (+/- 2 months) for patients, and at M36 (+/- 12 months) for asymptomatic carriers of pathogenic mutations. The aim of this research is to model ALS pathology using fibroblasts derived from the patients' skin biopsies.
Detailed Description
Amyotrophic Lateral Sclerosis (ALS) is the most common motor neuron disease in adults. This longitudinal study involves three cohorts of participants: patients with sporadic or hereditary ALS, asymptomatic individuals carrying pathogenic mutations responsible for ALS, and control subjects. In this study, a skin biopsy and blood sampling will be performed at the initial visit (M0), then at M12 (+/- 2 months) for patients, and at M36 (+/- 12 months) for asymptomatic carriers of pathogenic mutations. The aim of this research is to model ALS pathology using fibroblasts derived from the patients' skin biopsies.
Investigators
Eligibility Criteria
Inclusion Criteria
- •common to all 3 populations:
- •adults, both sexes
- •with written consent to participate in the study
- •affiliated to a social security scheme
- •ALS patients :
- •patients with ALS according to the revised El Escorial criteria :
- •with a hereditary form of ALS, defined by the presence of a family history of ALS or by the demonstration of a pathogenic mutation in the patient or
- •with a juvenile form of the disease, defined by onset of symptoms at less than 30 years of age or
- •with a sporadic form of ALS
- •Asymptomatic mutation carriers :
Exclusion Criteria
- •with a known skin disease (acne, atopic dermatitis, psoriasis, melanoma, skin carcinoma, rosacea, scabies; as referenced on http://dermato-info.fr/), which in the investigator's opinion constitutes a contraindication to skin biopsy
- •have a platelet count of less than 75,000/mm3 in a laboratory test less than 3 months old,
- •with a proven allergy to lidocaine or prilocaine,
- •Pregnant or breast-feeding women, or subjects under guardianship, curatorship or safeguard of justice.
- •Patient's condition which, in the opinion of the investigator, is incompatible with skin sampling or participation in the study.
- •Participation in a clinical trial (involving a drug) or other interventional research if this interferes with FIBRALS research.
Arms & Interventions
participants (SLA, healthy controls and asymptomatics)
Patients fulfilling the El Escorial criteria definite ALS or asymptomatics or Healthy controls
Intervention: biopsy
Outcomes
Primary Outcomes
Detection of cytoplasmic TDP-43, TIA1 and/or p62 aggregates in patient fibroblasts
Time Frame: 1 year
Detection of cytoplasmic TDP-43, TIA1 and/or p62 aggregates in patient fibroblasts subjected to various cellular stresses by immunofluorescence analysis.