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Determination of the Safety and Efficacy of Two Probiotic Strains

Not Applicable
Not yet recruiting
Conditions
Gastrointestinal Dysfunction
Interventions
Dietary Supplement: Placebo 1 to Probiotic A
Dietary Supplement: Placebo 2 to Probiotic B
Dietary Supplement: Probiotic B to Placebo 2
Dietary Supplement: Probiotic A to Placebo 1
Registration Number
NCT06466174
Lead Sponsor
The Archer-Daniels-Midland Company
Brief Summary

The goal of this study is to determine the safety and efficacy of two probiotic strains in adults with mild to moderate gastrointestinal discomfort. The main questions it aims to answer is whether 6-week administration of a probiotic can improve how the participant feels regarding their gut health. This is done by completing a weekly questionnaire and the results are compared between the 6 weeks the participants are on a placebo and the 6 weeks the participants are on the probiotic. Participants will also provide a blood sample, and stool sample at the start and end of each treatment. Similarly, the results from the blood samples and stool samples will be analysed to determine if there is any improvement in markers related to gut health.

Detailed Description

In this randomized, crossover, double-blind, prospective, placebo controlled study we aim to determine the safety and efficacy of two probiotic strains in adults with mild to moderate gastrointestinal discomfort. 100 healthy adults with subclinical mild to moderate gastrointestinal complaints (GSRS-IBS score 20-45 inclusive), 50 subjects per strain (test sequence). The GSRS-IBS (gastrointestinal symptom rating scale - irritable bowel syndrome) questionnaire ranges from 0-78 with a greater score indicating a higher occurrence of gastrointestinal symptoms. Safety: To evaluate adverse events that occur during the probiotic treatment period as compared to the placebo period. Incidence and duration of upper respiratory tract symptoms, urinary tract symptoms, gastrointestinal symptoms as primary outputs. Efficacy: To evaluate the effect of 6-week probiotic administration on gastrointestinal homeostasis using GSRS-IBS score as an index as compared to placebo. Following a screening period, participants will provide stool, urine (safety) and blood samples prior to commencing a 6 week course of either placebo or one of the probiotics. At this time participants will again provide blood and stool samples. Throughout the study participants will maintain a daily diary to note any unusual events and to report stool consistency using Bristol stool chart. The participants will also complete a once-weekly questionnaire (GSRS-IBS questionnaire) relating to gut health. There will then be a 3-week washout period where they will take no products. After these 3 weeks the participants will again provide a stool and blood samples, and as before maintain their daily diary and weekly questionnaire for 6 weeks while on the probiotic or placebo (they will have swapped at this point). After these 6 weeks the participants will again provide stool and blood samples.

Recruitment & Eligibility

Status
NOT_YET_RECRUITING
Sex
All
Target Recruitment
100
Inclusion Criteria
  1. Signed Informed consent form
  2. Healthy adults aged 18-65 years
  3. Subclinical mild to moderate gastrointestinal complaints (GSRS-IBS score 20-45 at baseline)
Exclusion Criteria
  1. Presence of a serious congenital anomaly or chronic medical condition that would contraindicate participation, including history of major gastrointestinal surgery, chronic gastrointestinal illness, abnormal intestinal anatomy or significant abdominal disorder
  2. Severe chronic illnesses
  3. Known immunodeficiency
  4. Use of immunosuppressive agents (corticosteroids, methotrexate...)
  5. Presence of severe immunodeficient family members as provided by subjects self-report
  6. Pregnancy, 6 months postpartum period or current breastfeeding
  7. Women of childbearing age planning pregnancy during the course of the study
  8. Actively participating in another clinical study, having completed a clinical study in the past 60 days or being in the exclusion period of a previous clinical study
  9. Not able to understand and comply with requirements of the study
  10. Use of probiotics, prebiotics, postbiotics, antibiotics within 3 weeks of study commencement

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Placebo 1 to Probiotic APlacebo 1 to Probiotic ASequence A1 (n=25): Placebo 1 administered daily for 42 days, followed by 21 days washout, then 1 x 10\^9 colony forming units (CFU) of Probiotic A administered daily for 42 days
Placebo 2 to Probiotic BPlacebo 2 to Probiotic BSequence B1 (n=25): Placebo 2 administered daily for 42 days, followed by 21 days washout and 42 days Probiotic formula: containing 1 x 10\^9 CFU of Probiotic B administered daily
Probiotic B to Placebo 2Probiotic B to Placebo 2Sequence B2 (n=25): 1 x 10\^9 CFU of Probiotic B administered daily for 42 days, followed by 21 days washout and 42 days Placebo 2 administered daily for 42 days
Probiotic A to Placebo 1Probiotic A to Placebo 1Sequence A2 (n=25): 1 x 10\^9 CFU of Probiotic A administered daily for 42 days, followed by 21 days washout, then Placebo 1 administered daily for 42 days
Primary Outcome Measures
NameTimeMethod
Change in GSRS-IBS scoreBaseline vs Week 6

Change in the GSRS-IBS score while participants are on the treatment as compared with placebo. GSRS-IBS ranges from 0-78 with a greater score indicating a higher occurence of gastrointestinal symptoms

Secondary Outcome Measures
NameTimeMethod
Duration of urinary tract infectionsBaseline vs Week 6

Duration of urinary tract infections, as captured by duration of urinary tract infections as reported by clinician/PI

Incidence of upper respiratory tract symptomsBaseline vs Week 6

Incidence of upper respiratory tract symptoms, as captured by incidence of upper respiratory tract as reported by clinician/PI

Incidence of urinary tract infectionsBaseline vs Week 6

Incidence of urinary tract infections, as captured by incidence of urinary tract infections as reported by clinician/PI

Duration of upper respiratory tract symptomsBaseline vs Week 6

Duration of upper respiratory tract symptoms, as captured by duration of upper respiratory tract as reported by clinician/PI

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