Identifying Molecular Determinants of Infertility in Men (MODIFY)
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Male Infertility
- Sponsor
- Institute for Human Development and Potential (IHDP), Singapore
- Enrollment
- 100
- Locations
- 1
- Primary Endpoint
- Semen/Sperm quantity analysis
- Status
- Completed
- Last Updated
- 10 months ago
Overview
Brief Summary
The purpose of this study is to understand the underlying mechanisms of infertility caused by unknown factors. The investigator propose to identify small non-coding RNA (sncRNA) biomarkers of infertility and advance towards developing a more accurate and robust approach for infertility diagnosis.
Detailed Description
The goal of the study is to identify small non-coding RNA signatures of infertility in men through the comparison of sperm sncRNA profiles from around 100 men (21-50 years of age). The participants will be classified into fertile and infertile groups by the conventional method. Through the project, the investigator intends to understand the underlying mechanisms of idiopathic infertility. By utilizing semen as liquid biopsy, through sperm sncRNA profiling and comparison (infertile vs fertile). The investigator proposes to identify the sncRNA (miRNAs, piRNAs and tRFs) as biomarkers of infertility. The investigator proposes to generate a validated signature of sncRNAs that will help in selecting high quality sperms for IVF.
Investigators
Candida Vaz
Principal Investigator, Senior research scientist
Institute for Human Development and Potential (IHDP), Singapore
Eligibility Criteria
Inclusion Criteria
- Not provided
Exclusion Criteria
- Not provided
Outcomes
Primary Outcomes
Semen/Sperm quantity analysis
Time Frame: 1 week
sperm count and concentration
Fertility classification
Time Frame: 1 week
Classification into fertile/infertile groups based on the fertility measures.
Semen/Sperm quality analysis
Time Frame: 1 week
semen pH, semen volume, sperm motility, sperm morphology, sperm vitality
Sperm RNA extraction and sequencing
Time Frame: 3 months
Sperm RNA extraction and sequencing
Secondary Outcomes
- Expression profiling of sncRNA(3-6 months)
- Comparison of the expression profiles(3-6 months)