Isolation of Cells From Biopsy Tissue to Aid in Kidney Repair
试验速览
- 阶段
- 1 期
- 状态
- 撤回
- 试验地点
- 2
- 主要终点
- Cell growth
研究概览
简要总结
Endothelial progenitor cells that reside in renal vasculature may be stimulated to initiate differentiation programs during episodes of injury. It is hypothesized that endothelial progenitor cells resident in the kidney can transition to a post-injury phenotype that promotes endothelial repair.
详细描述
Endothelial dysfunction is central to the pathophysiology of vascular ischemia, bacterial sepsis, toxin-induced thrombotic microangiopathy, and antibody-mediated kidney transplant rejection and often manifest with renal failure. With each of these diagnoses circulating components of the complement cascade bind to endothelial cells and induce disease progression through anaphylactic cellular messaging, monocyte homing, and direct cell membrane disruption. In response to injury during embryologic development avascular metanephric blastema initiate endothelial differentiation. Although circulating hematopoietic progenitor cells have shown therapeutic promise they incorporate into renal vasculature at very low density.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 85 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Only patients who have a clinical indication for kidney biopsy will be considered.
- •All subjects will be at least 18 years of age and may be of any gender.
- •Patients undergoing a native or kidney transplant biopsy will be considered.
排除标准
- •Patients not undergoing a kidney biopsy will be excluded.
结局指标
主要结局
Cell growth
时间窗: 1 week
Tissue will be isolated and cell viability will be documented by rate of colony growth over one week per patient enrolled.
次要结局
- Kidney disease progression(3 years)
研究者
Andrew Michael Siedlecki
Assistant Professor
Brigham and Women's Hospital