Reliability of longterm preservation of immunofluorescence slides: a prospective observational study
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 240
- 试验地点
- 1
- 主要终点
- Recording the intensity of the direct immunofluorescence slides in baseline and subsequent months and analyzing the data and calculating the survival of slides in percentage
研究概览
简要总结
Direct immunofluorescence has proven indispensable in the examination and identification of diverse immuno bullous diseases. Despite its widespread application, there is limited understanding of how well the fluorescent staining, particularly in skin specimens, can be preserved.Reviewing DIF slides can be important for re-evaluating a previously rendered diagnosis, either for an internal review or an outside consultation. Thus a reliable storage period for keeping IF slides, should be well established.
Out of 240 slides 80 are stored in deep freezers as controls, 80 in room temperature and 80 in cool temperature (22 degree C) in light proof packets and examined subsequently by dermatologist who was blind to initial diagnosis at 3rd , 6th and 12th months.
研究设计
- 研究类型
- Observational
入排标准
- 年龄范围
- 1.00 Month(s) 至 99.00 Year(s)（—）
- 性别
- All
入选标准
- •All DIF positive slides that is reported in the DIF lab.
排除标准
- 未提供
结局指标
主要结局
Recording the intensity of the direct immunofluorescence slides in baseline and subsequent months and analyzing the data and calculating the survival of slides in percentage
时间窗: Detection of tissue bound immune reactants by direct immunofluorescence microscopy at 3rd 6th 12th months.
次要结局
- 1.To compare the intensity of staining at baseline and at subsequent reading.(2.To compare the positivity at different temperature settings.)
研究者
Dr Swetha S Iyer
Kasturba Medical College Manipal