Cutibacterium Acnes in Bone and Joint Infections

• Conditions: Prosthesis and Bone Infections; Cutibacterium Acnes

• Registration Number: NCT03950063
• Lead Sponsor: CHU de Reims

Brief Summary

Prosthetic Joint Infections (PJIs) are increasing with the use of orthopedic devices on an ageing population. Cutibacterium acnes is a commensal organism that plays an important role in the ecosystem healthy human skin, yet this species is also recognized as a pathogen in foreign body infection: endocarditis, prostatitis and specifically in PJIs. C. acnes is able to escape the immune system. This phenomenon could reflect two bacterial behavior: the bacterial internalization by host cells and the biofilm formation.

Detailed Description

The aim of the study is to evaluate specific pathogenicity factors (ability to internalize bone cells and ability to form a biofilm) of bacteria derived from clinical strains of C. acnes isolated from bone infections on orthopedic material.

Study Timeline

• Status Verified: 2019-05
• Study First Submitted: 2019-05-13
• Study First Submitted QC: 2019-05-13
• Last Update Submitted: 2019-05-13
• Study First Posted: 2019-05-15
• Last Update Posted: 2019-05-15
• Study Start: 2019-06-15
• Primary Completion: 2020-06-15
• Study Completion: 2021-06-15

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 50

Inclusion Criteria

• bone infection with C. acnes,
• isolation of the strain,
• presence of a single species,
• conservation of a viable strain,
• patients over 18 years

Exclusion Criteria

Not provided

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Internalization capacity of C. acnes strains	Day 0	C. acnes strains will be cultured for 5 days in rich medium at 37°C in anaerobic conditions. Then, the bacteria will be co cultured with bone cells (osteoblast) and bacteria will be internalized by the bone cells. After killing bacteria stayed outside of the cells with an antibiotic and lysis of the cells, the number of bacteria internalized will be counted on agar plate.
Biofilm capacity of C. acnes strains	Day 0	C. acnes strains will be cultured for 5 days in rich medium at 37°C in anaerobic conditions. Then, the biofilm biomass will be evaluated by Crystal violet staining. The amount of biofilm will be evaluated by absorbance (optic density by spectrophotometry).

Trial Locations

Locations (1)

Damien JOLLY; 🇫🇷 Reims, France