Comparative Differences of Amino Acid Appearance with Milk Protein Ingestion with and Without Probiotic Supplementation
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 发起方
- 入组人数
- 30
- 试验地点
- 2
- 主要终点
- Rate of amino acid absorption
研究概览
简要总结
The purpose of this study is to examine the rate and concentration of amino acid absorption after a three-week pattern of milk protein supplementation with and without the addition of a single strain probiotic.
详细描述
The study will be conducted using a randomized, double-blind, crossover study design. Thirty healthy men and women between 18 - 35 years of age will be recruited to participate in this study. Two supplementation protocols that each span three weeks will be completed and separated with a washout period of three weeks. Participants will be assigned in a randomized, double-blind, crossover fashion to ingest a single daily 25-gram dose of a powder milk protein and placebo or a single daily 25-gram dose of a powder milk protein plus a single strain probiotic. Upon arrival for each study visit, participants will have their resting heart rate, blood pressure, body mass, height, and body composition measured. On the day of experimental testing, subjects will arrive to the laboratory in a fasted state. Subjects will rest semi-supine for multiple blood sampling. Following baseline sampling, participants will ingest their respective supplement plus a standard bolus of milk protein (powder form, mixed with 12 fl oz of cold water). Thereafter, blood samples will be taken at 30, 60, 90, 120, 180, and 240 minutes post-ingestion. Study participants will be provided an additional three-week supply of the alternative treatment to begin after observing a three-week washout. After three weeks of supplementation, study participants will return to the laboratory for their remaining testing visit.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Double (Participant, Investigator)
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Participants will be male or female between the ages of 18-35 years;
- •Participants have not been consuming any nutritional supplements for the preceding 6-week period;
- •Participants have not been consuming any ergogenic aids for the preceding 6-week period;
- •Participant has provided written and dated informed consent to participate in the study;
- •Participant is willing and able to comply with the protocol;
- •Participant is apparently healthy and free from disease, as determined by a health history questionnaire;
排除标准
- •Participant is allergic to any ingredient in the nutritional supplement or placebo;
- •Participant is not pregnant or trying to become pregnant, nor breast feeding;
- •Participant reports any unusual adverse events associated with this study that in consultation with the study investigators or their doctor recommends removal from the study;
- •Participant currently ingests nicotine or has quit within the last 6 months;
结局指标
主要结局
Rate of amino acid absorption
时间窗: Day 0, Day 22, Day 65
To determine if the addition of a probiotic to milk protein ingestion changes the rate (in minutes) to which amino acids are absorbed into our bloodstream.
Concentration of amino acid absorption
时间窗: Day 0, Day 22, Day 65
To determine if the addition of a probiotic to milk protein ingestion changes the extent (concentration in millimoles/Liter) to which amino acids are absorbed into our bloodstream.
次要结局
- Comprehensive metabolic panel(Day 0, Day 22, Day 65)
- Blood CBC panel(Day 0, Day 22, Day 65)