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Perioperative Vitamin C Lung Transplant

Phase 2
Withdrawn
Conditions
Primary Graft Dysfunction
Lung Transplant; Complications
Interventions
Registration Number
NCT04505878
Lead Sponsor
University of Wisconsin, Madison
Brief Summary

This study is being done to determine if parenterally administered ascorbic acid (Vitamin C) given at the time of lung transplant is safe. Vitamin C may be an effective intervention towards primary graft dysfunction (PGD). The study will enroll 69 participants who consent to the intervention. Participants who do not consent to the intervention will be treated according to standard-of-care, but may choose to be consented to have their data retrospectively reviewed. Based on our consent rate, this group may include 40-70 participants. Participants will be on study for up to 12 months.

Detailed Description

PGD is a frequent and severe outcome that impacts both short- and long-term outcomes after lung transplantation. Major pathophysiologic contributors include ischemia and reperfusion injury, mitochondrial dysfunction and endothelial failure. No directed therapy exists. Vitamin C is a first-line antioxidant that also acts at the endothelium and mitochondria to decrease permeability and leak, inhibit mitochondrial dysfunction and improve ischemia and reperfusion. When combined with steroids, part of standard care for lung transplant recipients, these effects may be enhanced and synergistically inhibit instigators of patient injury. A pilot trial will ensure safety of this potential intervention and guide future research into this important outcome measure. It will be readily received in the literature.

For the present study, vitamin C will be administered parenterally at a dose of 1,500 mg every 6 hours, a dose that is widely accepted and used in other clinical contexts where the drug is studied, such as sepsis. This will predictably reconstitute levels and achieve supratherapeutic benefit towards oxidant scavenging, while avoiding the potential pro-oxidant effects seen at exceedingly high doses. To this end, the investigators will exclude patients where the standard dosing of vitamin C will exceed 100 mg/kg/day (excluding patients \<60 kg). Dosing will continue through post-operative day (POD) 3 to effectively assess for the impact of vitamin C on PGD.

Primary Objectives

* To assess whether parenterally administered ascorbic acid (vitamin C) is safe in the lung transplant population

* To estimate adherence to ascorbic acid administration protocol in this study population and to identify obstacles to feasibility of future trials using this protocol

Secondary Objectives

* To assess whether parenterally administered ascorbic acid (vitamin C) may decrease the rate and severity of PGD after lung transplant

* To establish the incidence of vitamin C and vitamin B1 (thiamine) deficiencies in the lung transplant population, and the responsiveness of vitamin C levels to our selected parenteral therapy

* To identify interventions that will optimize the post-operative wellbeing of patients receiving lung transplants by decreasing primary graft dysfunction (short and intermediate-to-long term

Stop Criteria

* Anuria x 3-hours

* Moderate, Grade 2 AKI (doubling of baseline creatinine)

* An acute, unexplained hemoglobin drop of \>2 mg/dL

Recruitment & Eligibility

Status
WITHDRAWN
Sex
All
Target Recruitment
Not specified
Inclusion Criteria
  • Participant is scheduled for lung transplantation
Exclusion Criteria
  • Non-English speaking
  • Subject is known or believed to be pregnant
  • Subject is a prisoner.
  • Subject has impaired decision-making capacity.
  • Subject has known allergy to vitamin C.
  • Subject has history of nephrolithiasis, oxalosis or hyperoxaluria. (Cystic Fibrosis patients are at risk of occult oxalosis / hyperoxaluria, therefore they will also be excluded from the study.)
  • Glucose-6-phosphate dehydrogenase (G6PD) deficiency
  • Sickle cell anemia
  • Heredity hemochromatosis
  • Baseline creatinine >2 mg/dL or any current kidney injury
  • Weight <60 kg
  • Vitamin C supplement use or administration (including HAT therapy) within the last month prior to transplantation
  • Current enrolment in another research study
  • Not suitable for study participation due to other reasons at the discretion of the investigators.

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Vitamin C ArmVitamin CAscorbic Acid will be administered at a dose of 1500 mg in 100 mL of saline over 30 minutes intravenously once every 6 hours for a total of 72 hours
Primary Outcome Measures
NameTimeMethod
Incidence and Severity of Kidney Injury POD 4Post Operative Day 4

The primary endpoint is the safety of the vitamin C intervention. This will be measured by the incidence and severity of kidney injuries on POD 1, POD 2, POD 3, POD 4, and POD 7. Acute kidney injury (AKI) is defined as any creatinine (Cr) that is 1.5-times the participant's baseline / immediate preoperative creatinine. Severity escalates with increasing ratios: Mild, stage 1 AKI (Cr 1.5-1.99x baseline); Moderate, stage 2 AKI (Cr 2-2.9x baseline); and Severe, stage 3 AKI (Cr \>3x baseline or any initiation of dialysis).

Incidence and Severity of Kidney Injury POD 7Post Operative Day 7

The primary endpoint is the safety of the vitamin C intervention. This will be measured by the incidence and severity of kidney injuries on POD 1, POD 2, POD 3, POD 4, and POD 7. Acute kidney injury (AKI) is defined as any creatinine (Cr) that is 1.5-times the participant's baseline / immediate preoperative creatinine. Severity escalates with increasing ratios: Mild, stage 1 AKI (Cr 1.5-1.99x baseline); Moderate, stage 2 AKI (Cr 2-2.9x baseline); and Severe, stage 3 AKI (Cr \>3x baseline or any initiation of dialysis).

Incidence of New Dialysis Initiationup to Post Operative Day 7
Incidence and Severity of Kidney Injury Post Operative Day (POD) 1Post Operative Day 1

The primary endpoint is the safety of the vitamin C intervention. This will be measured by the incidence and severity of kidney injuries on POD 1, POD 2, POD 3, POD 4, and POD 7. Acute kidney injury (AKI) is defined as any creatinine (Cr) that is 1.5-times the participant's baseline / immediate preoperative creatinine. Severity escalates with increasing ratios: Mild, stage 1 AKI (Cr 1.5-1.99x baseline); Moderate, stage 2 AKI (Cr 2-2.9x baseline); and Severe, stage 3 AKI (Cr \>3x baseline or any initiation of dialysis).

Incidence and Severity of Kidney Injury POD 2Post Operative Day 2

The primary endpoint is the safety of the vitamin C intervention. This will be measured by the incidence and severity of kidney injuries on POD 1, POD 2, POD 3, POD 4, and POD 7. Acute kidney injury (AKI) is defined as any creatinine (Cr) that is 1.5-times the participant's baseline / immediate preoperative creatinine. Severity escalates with increasing ratios: Mild, stage 1 AKI (Cr 1.5-1.99x baseline); Moderate, stage 2 AKI (Cr 2-2.9x baseline); and Severe, stage 3 AKI (Cr \>3x baseline or any initiation of dialysis).

Incidence and Severity of Kidney Injury POD 3Post Operative Day 3

The primary endpoint is the safety of the vitamin C intervention. This will be measured by the incidence and severity of kidney injuries on POD 1, POD 2, POD 3, POD 4, and POD 7. Acute kidney injury (AKI) is defined as any creatinine (Cr) that is 1.5-times the participant's baseline / immediate preoperative creatinine. Severity escalates with increasing ratios: Mild, stage 1 AKI (Cr 1.5-1.99x baseline); Moderate, stage 2 AKI (Cr 2-2.9x baseline); and Severe, stage 3 AKI (Cr \>3x baseline or any initiation of dialysis).

Secondary Outcome Measures
NameTimeMethod
Participant Vitamin C LevelsBaseline, Post Operative Day 1, Post Operative Day 2, Post Operative Day 3
Participant Thiamine LevelsBaseline, Post Operative Day 1, Post Operative Day 2, Post Operative Day 3
Incidence of Primary Graft Dysfunction (PGD)up to Post Operative Day 7

Primary Graft Dysfunction is defined as chest x-ray (CXR)-infiltrates with or without depressed oxygenation function, assessed by the "PF-Ratio," which is the PaO2 / FiO2.

Incidence and Severity of PGD on POD 3Post Operative Day 3

Primary Graft Dysfunction is defined as chest x-ray (CXR)-infiltrates with or without depressed oxygenation function, assessed by the "PF-Ratio," which is the PaO2 / FiO2. Severity is defined as: PGD Grade 1 = CXR findings and any PF Ratio \> 300; PGD Grade 2 = CXR findings and PF Ratio 200-300; and PGD Grade 3 = CXR findings and PF Ratio \<200.

Tacrolimus LevelsPost Operative Days 2, 3, 4, and 7
Tacrolimus DosesPost Operative Days 4 and 7
Post-Operative Well Being: Mortalityat Post Operative Day 30 and Post Operative Day 90 via chart review
Post-Operative Well Being: Atrial Fibrillationup to Post Operative Day 7
Post-Operative Well Being: ICU Length of Stayup to Post Operative Day 30 (chart review)
Post-Operative Well Being: Hospital Length of Stayup to Post Operative Day 90 (chart review)
Post-Operative Well Being: Nadir Cardiac Indexup to 72 hours post op
Post-Operative Well Being: Peak Pulmonary Artery Systolic Pressureup to 72 hours post op
Post-Operative Well Being: Peak Pulmonary Artery Diastolic Pressureup to 72 hours post op
Post-Operative Well Being: Duration of Inotrope Needup to 72 hours post op
Post-Operative Well Being: Duration of Vasopressorup to 72 hours post op
Post-Operative Well Being: Total Dose of Vasopressorup to 72 hours post op
Post-Operative Well Being: Daily Crystalloid Volumeup to 72 hours post op

for 0-24h, 24-48h and 48-72h; total volume balance at 72-hours

Post-Operative Well Being: Daily Blood Product Transfusion Volumeup to 72 hours post op

for 0-24h, 24-48h and 48-72h; total volume balance at 72-hours

Post-Operative Well Being: Daily Chest Tube Output Volumeup to 72 hours post op

for 0-24h, 24-48h and 48-72h; total volume balance at 72-hours

Post-Operative Well Being: Duration of Post-Operative Mechanical Ventilationup to Post Operative Day 7
Post-Operative Well Being: PaO2 / FIO2 ratiosPost Operative Day 1, Post Operative Day 2, Post Operative Day 3

The PF Ratio assesses the lungs' ability to oxygenate the blood. It is defined as the ratio of the partial pressure of oxygen in the arteries (PaO2 in mmHg) to the fractional inspired oxygen content from the ventilator (FiO2 in %).

Post-Operative Well Being: Time to Clearance of Lactateup to Post Operative Day 3

"Clearance" is defined as a lactate \<1 mmol/L.

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