Immunogenicity of 7-Valent Pneumococcal Conjugate Vaccine in Lung Fluid of Adults With and Without HIV Infection
- Conditions
- PneumoniaMeningitisInvasive Pneumococcal DiseaseHIV
- Registration Number
- NCT00371878
- Lead Sponsor
- Royal Liverpool University Hospital
- Brief Summary
Lung immune responses are regulated independently of systemic responses. Injected vaccines may induce optimal responses in blood but not at mucosal surfaces. We compared the responses in serum and lung fluid to injected pneumococcal conjugate vaccine.
- Detailed Description
We tested the hypothesis that conjugate vaccine offered less protection against pneumonia due to a reduced mucosal response compared to serum. We further hypothesized that this response would be further compromised with HIV co-infection\[32\] due to lack of local CD4 lymphocyte support and an altered alveolar milieu. We measured pneumococcal capsular specific immunoglobulin responses to 7-valent conjugate vaccine in both lung fluid and serum from healthy HIV infected and uninfected volunteers, together with flow cytometric assessment of the relative numbers and phenotypes of BAL T lymphocyte, B lymphocyte and macrophages.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 40
- healthy volunteer adults
- pregnant, recent illness, previous vaccination, asthma
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- SINGLE_GROUP
- Primary Outcome Measures
Name Time Method Immunoglobulin titres in bronchoalveolar fluid
- Secondary Outcome Measures
Name Time Method Immunoglobulin titres in serum Lung lymphocyte phenotype HIV viral load
Trial Locations
- Locations (1)
Wellcome Trust Laboratories and Dept of Medicine, Queen Elizabeth Central Hospital
🇲🇼Blantyre, Malawi