Biochemical and Genetic Mechanisms of Acute Clinical Pain During Bone Marrow Aspiration
试验速览
- 阶段
- 不适用
- 状态
- 撤回
- 主要终点
- Differences over time between whole blood gene expression in the peripheral blood of healthy subjects undergoing an acute pain stimulus.
研究概览
简要总结
Background:
- Many people feel pain during and after bone marrow aspiration (collection). Studies suggest that pain may be related to different kinds of inflammation, or the presence of nitric oxide (a normally occurring gas) in the body. Researchers want to study nitric oxide levels in the blood and breath before and after bone marrow collection. They will look at how these levels relate to feelings of pain.
Objectives:
- To better understand pain related to having a bone marrow collection.
Eligibility:
- Individuals at least 18 years of age who are enrolled in a study that requires bone marrow collection.
Design:
- Participants will be screened with a physical exam, medical history, and blood sample.
- Participants will provide study blood and breath samples to check nitric oxide levels. Participants will also have a test to measure skin sensitivity to heat and touch.
- During the collection, participants will keep track of their pain levels.
- After the collection, participants will keep track of any pain medications they take. They will also record if and when they eat any foods that contain nitrates for about 24 hours.
详细描述
Abstract:
This project will explore the biochemical mechanisms associated with acute pain produced by bone marrow aspiration in 36 healthy subjects by : 1) Evaluation of any strong relationships of NO metabolism (as measured by plasma nitrite, nitrosated proteins, cyclic GMP, plasma hemoglobin, reticulocytes, as well as markers of inflammation) with the levels of acute pain in healthy subjects and; 2) Identification of differentially expressed genes and proteins as markers for the nature and severity of pain from bone marrow aspiration. Elucidation of these relationships may identify novel targets for intervention leading to attenuation of pain, improved treatment and less impact on quality of life (QOL) and may identify potential mechanisms for the role of NO in other inflammatory or pain syndromes.
Objectives:
The first objective of the study is to characterize genetic expression during acute pain and pain free phases pre and post bone marrow aspiration (BMA). The second objective is to measure at multiple time points levels of exhaled Nitric Oxide (eNO) and other biomarkers in subjects during pre- and post BMA. The third objective is to evaluate acute and experimental pain perception in these subjects.
Population:
研究设计
- 研究类型
- Observational
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- 未提供
排除标准
- 未提供
结局指标
主要结局
Differences over time between whole blood gene expression in the peripheral blood of healthy subjects undergoing an acute pain stimulus.
次要结局
- Quantification of pain sensory perception in healthy subjects
- Quantification and differences in laboratory correlates (particularly plasma nitrite, nitrosated proteins, and cyclic GMP) in health subjects pre- and post-BMA.