Dose Ranging Study of ART621 in Subjects Diagnosed With Rheumatoid Arthritis Taking Methotrexate
- Registration Number
- NCT00928317
- Lead Sponsor
- Arana Therapeutics Ltd
- Brief Summary
The purpose of this clinical trial is to assess the safety, efficacy, tolerability, immunogenicity and pharmacokinetics of 3 dose levels of ART621 in the treatment of rheumatoid arthritis.
- Detailed Description
Despite being effective in approximately 60% of subjects, there are limitations to existing anti-TNF therapies especially in relation to immunogenicity, safety and administration. In addition, due to their high molecular weight, currently marketed products are largely confined to the blood stream.
ART621 is an anti-TNF molecule that contains 2 identical domain "antibodies" that have the binding activity of a full antibody but with a substantially smaller molecular size. The molecular weight of approximately half that of full size antibodies is predicted to, a) have improved tissue penetration and, b) to be less immunogenic than full size antibodies.
This clinical trial is designed to assess the safety, efficacy, tolerability, immunogenicity and pharmacokinetics of ART621 when administered with an intravenous loading dose followed by subcutaneous administration every week.
Recruitment & Eligibility
- Status
- TERMINATED
- Sex
- All
- Target Recruitment
- 13
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Provision of a valid written informed consent.
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Male or female subjects ≥ 18 and ≤ 80 years old.
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Women of childbearing potential, or men of fathering potential, must be using adequate (in the investigator's opinion) birth control measures (e.g. abstinence, oral contraceptives, intrauterine device, barrier method with spermicide, or surgical sterilisation) during the study. Female subjects of childbearing potential must test negative for pregnancy prior to enrolling in the study. Post menopausal (cessation of menses for more than 2 years) women are eligible for this study.
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Diagnosis of RA according to the revised (1987) American College of Rheumatology criteria for at least 6 months and no longer than 3 years prior to screening.
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Meet ACR functional class criteria I, II or III.
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Have active RA at the time of screening and at baseline, defined as ≥ 6 swollen joints and ≥ 6 tender joints (from 68 joint count) together with at least 2 of the following 3 criteria:
- CRP level ≥ 1.5 mg/dl;
- ESR by Westergren method ≥ 28 mm in the first hour; or
- morning stiffness ≥ 45 minutes.
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At least one of the following should be present at screening:
- documented history or current presence of positive rheumatoid factor;
- presence of serum anti-CCP antibodies; or
- screening radiographic erosion
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Have been tolerating concomitant methotrexate (oral or subcutaneous) for at least 3 months prior to screening and on a stable dose between 10-25 mg per week for at least 6 weeks prior to the first study dose. The route of administration must also be stable. Use of methotrexate dose of 25-50 mg every 2 weeks is also acceptable. (Other DMARDs taken concomitantly with methotrexate are not allowed. Those subjects concomitantly receiving additional DMARDs with methotrexate may enter the study by stopping the additional DMARD at least 4 weeks prior to first study dose).
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If using the following medication, the subject must be on a stable dose for the 4 weeks prior to the first study dose and maintain that dose throughout the study:
- oral corticosteroids, equivalent to ≤ 10 mg of prednisone/day.
- one nonsteroidal anti-inflammatory drug (NSAID).
- 3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitors (statins) or fibrates (see Section 7.1 for acceptable doses).
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Does not have active or latent TB according to eligibility assessment and screening rules (see Section 8.3.1).
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Is willing and able to comply with study visits and other protocol requirements.
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Pregnant, nursing, or planning a pregnancy (both men and women) within 9 months of enrolment.
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Subjects weighing > 100 kgs.
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Screening laboratory tests:
- hemoglobin ≤ 8.0 gm/dl
- white blood cells ≤ 3.0 x103 cells/µl
- neutrophils ≤ 1.5 x 103 cells/µl
- platelets ≤100 x 103 cells/µl
- serum transaminase level (AST and ALT) ≥ 2 times upper limit of normal (ULN)
- serum creatinine ≥ 0.15 mmol/l
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Subjects with diagnosis of juvenile arthritis or other inflammatory or autoimmune diseases that might confound the evaluations of benefit from ART621 such as ankylosing spondylitis, systemic lupus erythematosus and Lyme disease.
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Subjects who have previously failed to respond to any oral or injectable anti-TNFα therapy or subjects who have had to stop anti-TNFα therapy for safety reasons. Subjects who have successfully responded to anti-TNFα therapy in the past (but discontinued for reasons other than safety or lack of efficacy) > 6 months prior to study day one may enrol. Patients who have participated in a previous anti-TNFα therapy study are eligible if they are confirmed to have received placebo.
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Subjects who have previously received the following anti-rheumatic drugs: interleukin-1 receptor antagonist [anakinra], rituximab, anti-CD4 antibody, abatacept, thalidomide, p38 MAP kinase inhibitor and other agents (other than those listed in Section 7.3).
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Subjects who have undergone plasmapheresis within 6 months prior to randomisation.
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Have received intraarticular, intramuscular, or intravenous corticosteroids, including intramuscular adrenocorticotropic hormone, during the 4 weeks prior to the first study dose, or non-stable doses of oral steroids.
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Subjects with a history of any clinically significant adverse reaction to murine or chimeric proteins, including serious allergic reactions.
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Subjects with Felty's syndrome or a history of Felty's syndrome.
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Subjects who have received or are expecting to receive any live virus or bacterial vaccinations within 1 month before first study dose, during the study, or up to 3 months after the study dose.
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Subjects with a history of, presence of, or at high risk of serious infection including:
- history of active TB, or positive Mantoux test or QuantiFERON Gold test or chest x-ray suggestive of active or healed TB or positive contact history with a subject with active TB within the past 3 months. If subjects have a positive Mantoux test but a negative QuantiFERON Gold test, they may be enrolled.
- a serious infection during the 3 months prior study entry (hospitalised or received IV antibiotics for an infection).
- chronic or recurrent infectious disease.
- systemic fungal infections
- opportunistic infection within 3 months prior to screening (refer to 1993 CDC Classification System for HIV Infection).
- subjects known, or suspected, to be infected with HIV, hepatitis B, or hepatitis C.
- subjects with planned joint replacement surgery or a history of infected joint prosthesis or who have received antibiotics for a suspected infection of a joint prosthesis if that prosthesis has not been removed or replaced.
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Subjects with a known history of demyelinating diseases such as multiple sclerosis or optic neuritis.
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Subjects with evidence of severe, progressive, or uncontrolled renal, hepatic, hematologic, gastrointestinal, endocrine, pulmonary, cardiac, neurologic, psychiatric, or cerebral disease.
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Concurrent CHF, including medically controlled, asymptomatic CHF or ECG findings suggestive of CHF.
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Subjects receiving cytotoxic drugs including cyclophosphamide, cyclosporine, or alkylating agents within 6 months prior to first study dose.
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Known history or evidence of malignancy, lymphoproliferative or neoplastic disease with the exception of successfully treated basal or squamous cell carcinoma of the skin or cervical intraepithelial neoplasia.
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Subjects who have undergone organ transplant (with exception of a corneal transplant more than 3 months prior to screening).
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Subjects previously enrolled in this study, currently participating in another investigational study or treated with any investigational drug within the previous 3 months or within 5 half-lives, whichever is greater, prior to first study dose.
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Any other clinically significant disease or disorder or factors such as substance abuse which in the opinion of the investigator make the subject ineligible for participation in this study.
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description ART621 B ART621 ART621 1.5 mg/kg per week ART621 C ART621 ART621 3.0mg/kg per week ART621 A ART621 ART621 0.75mg/kg per week Placebo arm Placebo -
- Primary Outcome Measures
Name Time Method Efficacy of ART621 on the signs and symptoms of moderate to severe RA in subjects concomitantly taking methotrexate as assessed by the proportion of subjects achieving an ACR20 response. 12 weeks
- Secondary Outcome Measures
Name Time Method Dose-response relationship of ART621 against the signs and symptoms of moderate to severe RA as assessed by additional efficacy, safety and QoL parameters. 12 weeks Immunogenicity profile of ART621 as assessed by development of HAHAs. 16 weeks Plasma concentration versus time profile and population PK of ART621 in subjects with RA. 16 weeks Effect of ART621 on immunological parameters and other disease biomarkers. 12 weeks
Trial Locations
- Locations (45)
Nemocnice Jihlava
🇨🇿Jihlava, Czech Republic
ARTHROMED s. r. o.
🇨🇿Pardubice, Czech Republic
Fakultni nemocnice Plzen
🇨🇿Plzen - Bory, Czech Republic
Nizams Institute of Medical Sciences
🇮🇳Hyderabaad, Andh Prad, India
King George Hospital
🇮🇳Vishakhapattanam, Andh Prad, India
M.S. Ramaiah Memorial Hospital
🇮🇳Bangalore, Karna, India
NZOZ Centrum Osteoporozy i Chorob Kostno-Stawowych
🇵🇱Bialystok, Poland
St. John's Medical College Hospital
🇮🇳Bangalore, Karna, India
Hospital Ipoh
🇲🇾Perak, Malaysia
Krishna Institute of Medical Sciences
🇮🇳Secunderabad, Andh Prad, India
University Malaya Medical Centre
🇲🇾Kuala Lumpur, Malaysia
Samodzielny Publiczny ZOZ w Dzialdowie
🇵🇱Dzialdowo, Poland
SPSK nr 1 im. Tadeusza Sokolowskiego PAM
🇵🇱Szczecin, Poland
Revmatologicky ustav
🇨🇿Praha 2, Czech Republic
Apollo Hospital Educational and Research Foundation
🇮🇳Hyderabaad, Andh Prad, India
Centre for Rheumatic Diseases
🇮🇳Pune, Mahara, India
Medyczne Centrum Hetmanska
🇵🇱Poznan, Poland
Putra Medical Centre
🇲🇾Alor Setar, Kedah, Malaysia
Sanjay Gandhi Postgraduate Institute
🇮🇳Lucknow, Uttar Prad, India
Sarawak General Hospital
🇲🇾Sarawak, Malaysia
Nzoz Reumed
🇵🇱Lublin, Poland
Centrum Medyczne OSTEOMED
🇵🇱Warszawa, Poland
Instituto Medico CER
🇦🇷Quilmes, Bue, Argentina
Revmatologicka ambulance
🇨🇿Bruntal, Czech Republic
K. M. C. Hospital
🇮🇳Mangalore, India
Tampa Medical Group P.A.
🇺🇸Tampa, Florida, United States
Arthritis Center
🇺🇸Springfield, Illinois, United States
Instituto Medico Especializado IME
🇦🇷Buenos Aires, BUE, Argentina
ILAIM-CEOM Inst. Latinoamericano de Inv. Medicas
🇦🇷Cordoba, CRD, Argentina
Sanatorio Parque S.A.
🇦🇷Rosario, SFE, Argentina
Westroads Medical Group
🇺🇸Omaha, Nebraska, United States
Physician Research Collaboration, LLC
🇺🇸Lincoln, Nebraska, United States
Centro Medico Privado de Reumatologia
🇦🇷San Miguel de Tucuman, Argentina
Centro de Investigaciones Reumatológicas
🇦🇷Tucuman, TUC, Argentina
IMAI Research - Instituto Medico de Asistencia y Investigaci
🇦🇷Calle French, Argentina
Lyell McEwin Hospital
🇦🇺Elizabeth Vale, New South Wales, Australia
Coast Joint Care
🇦🇺Maroochydore, Queensland, Australia
The Queen Elizabeth Hospital
🇦🇺Woodville, South Australia, Australia
Apollo Hospitals Educational and Research Foundation
🇮🇳Madurai, Tamilnadu, India
C G M Research Trust, The Princess Margaret Hospital
🇳🇿Christchurch, New Zealand
Ctro Polivalente de Asist e Invest Clinica CER
🇦🇷San Juan, SJN, Argentina
Arthritis Northwest, PLLC
🇺🇸Spokane, Washington, United States
Arthritis Clinic
🇺🇸Racine, Wisconsin, United States
CAICI
🇦🇷Rosario, SFE, Argentina
Georgetown Arthritis Centre
🇦🇺Sydney, New South Wales, Australia