An ambispective comparative study of transcriptomic profiling in early and advanced stages of gallbladder adenocarcinoma
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 发起方
- 入组人数
- 40
- 试验地点
- 1
研究概览
简要总结
Gallbladder cancer (GBC) is an aggressive and often fatal disease, especially common in northern India. Most patients are diagnosed at an advanced stage when surgery is not possible, and current
treatments offer limited benefit. To improve outcomes, it is important to understand the molecular changes that drive cancer progression. This study aims to identify differential gene expression in early-stage and advanced-stage gallbladder cancer using a method called transcriptome analysis. RNA will be extracted from about 40 tissue samples (including early-stage, advanced-stage, and normal gallbladder tissues) collected from patients at HBCH and MPMMCC, Varanasi, using Next Generation Sequencing (NGS), researchers will map and compare gene expression patterns across these samples. The results are expected to identify key genes and biological pathways involved in the development and spread of gallbladder cancer. This knowledge could help discover new biomarkers for early detection and targets for better treatment, ultimately improving patient care.
研究设计
- 研究类型
- Observational
入排标准
- 年龄范围
- 40.00 Year(s) 至 80.00 Year(s)（—）
- 性别
- All
入选标准
- •Inclusion criteria for gallbladder adenocarcinoma:
- •In-house resected specimens of treatment-naive gall bladder adenocarcinomas received from Department of Surgical Oncology, MPMMCC Varanasi.
- •Histopathologically diagnosed treatment-naive gallbladder adenocarcinoma samples from patients presented with early stages (n=15) (upto T2 stage) and in advanced stages (n=15) (beyond T2).
- •Formalin fixed paraffin-embedded (FFPE) tissues of gallbladder adenocarcinomas with adequate tumor content will be taken up for transcriptomic analysis.
- •will be taken, those contain adequate tumor tissue, for transcriptomic analysis.
- •Inclusion criteria for controls :
- •In-house resected specimens of cases with benign gallbladder pathology/ uninvolved gallbladder (including chronic cholecystitis, specimen taken out for pancreatico-duodenectomy specimens, received from Department of Surgical Oncology, MPMMCC Varanasi.
排除标准
- •Exclusion criteria for gallbladder adenocarcinoma:
- •Patients who are treated with Neoadjuvant chemotherapy and/or radiotherapy before operative procedure.
- •FFPE tissues in which tumor tissue is inadequate for NGS testing.
- •Biopsy/resection specimens of gall bladder coming from outside will not be included in this study.
- •(Outside processed blocks) Exclusion criteria for controls :
- •Histologically diagnosed adenocarcinoma or any other malignancy.
- •Benign cases, that shows areas of necrosis, reaction atypia, dysplasia or atrophied gallbladder mucosa will not be included in this study.
研究者
Dr. Shashikant C U Patne
MPMMCC/HOMI BHABHA CANCER HOSPITAL VARANASI