Searching for Non-invasive Magnetic Resonance-based Markers for CrAT Protein Activity and Carnitine Availability in Skeletal Muscle
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 13
- 试验地点
- 1
- 主要终点
- Skeletal muscle PCr on-kinetic during exercise and carnitine acetyltransferase (CrAT) protein content/activity
研究概览
简要总结
This study aims to measure skeletal muscle metabolism in vivo during exercise using the non-invasive Magnetic Resonance Spectroscopy (MRS) technique. Specifically, phosphocreatine (PCr) kinetic and Acetylcarnitine levels will be determined at resting, during exercise and during the recovery post exercise. The target population is adult healthy men, with a wide range of maximal physical capacity.
详细描述
Skeletal muscle mitochondrial activation during the transition from resting metabolism to exercise may modulated by CrAT activity. Furthermore, dynamic changes in acetylcarnitine during the transition from exercise to resting metabolism (acetylcarnitine off-kinetic) may also reflect the action of CrAT protein. To date, the only possibility to get information on CrAT activity is by taking a muscle biopsy and perform enzyme activity assays ex vivo. By dynamic MRS scanning, it might be possible to deduce information that reflects CrAT activity, thereby making the biopsy sampling unnecessary.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Males, Generally healthy
- •Age 18-40 years old
- •BMI 18 - 25 kg/m2
- •No medication use that interfere with the aims of the study
- •Stable level of daily physical activity or training for at least 6 months
排除标准
- •Regular smokers, drug abuse
- •Participation in other studies
- •Weight gain/loss > 3 kg in the last 6 months
- •Contraindications for MRS scans:
- •Vegetarian eating behavior
结局指标
主要结局
Skeletal muscle PCr on-kinetic during exercise and carnitine acetyltransferase (CrAT) protein content/activity
时间窗: MRS+Exercise test day 2
The correlation between skeletal muscle PCr on-kinetic during one-legged exercise and CrAT protein activity will be determined.
次要结局
- Skeletal muscle acetylcarnitine recovery after one-legged exercise and carnitine acetyltransferase (CrAT) protein content/activity(MRS+Exercise test day 2)
- Skeletal muscle oxygen availability(MRS test day 1)
- Skeletal muscle CrAT protein activity, from muscle biopsy, and PCr breakdown rate during one-legged exercise(MRS+Exercise test day 1)
- Skeletal muscle acetylcarnitine formation capacity upon exercise and total carnitine availability in skeletal muscle(MRS+Exercise test day 2)
- Skeletal muscle mitochondrial function in vitro(MRS+Exercise test day 2)