Searching for Non-invasive Magnetic Resonance-based Markers for CrAT Protein Activity and Carnitine Availability in Skeletal Muscle
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Metabolism and Nutrition Disorder
- Sponsor
- Maastricht University
- Enrollment
- 13
- Locations
- 1
- Primary Endpoint
- Skeletal muscle PCr on-kinetic during exercise and carnitine acetyltransferase (CrAT) protein content/activity
- Status
- Completed
- Last Updated
- 5 years ago
Overview
Brief Summary
This study aims to measure skeletal muscle metabolism in vivo during exercise using the non-invasive Magnetic Resonance Spectroscopy (MRS) technique. Specifically, phosphocreatine (PCr) kinetic and Acetylcarnitine levels will be determined at resting, during exercise and during the recovery post exercise. The target population is adult healthy men, with a wide range of maximal physical capacity.
Detailed Description
Skeletal muscle mitochondrial activation during the transition from resting metabolism to exercise may modulated by CrAT activity. Furthermore, dynamic changes in acetylcarnitine during the transition from exercise to resting metabolism (acetylcarnitine off-kinetic) may also reflect the action of CrAT protein. To date, the only possibility to get information on CrAT activity is by taking a muscle biopsy and perform enzyme activity assays ex vivo. By dynamic MRS scanning, it might be possible to deduce information that reflects CrAT activity, thereby making the biopsy sampling unnecessary.
Investigators
Eligibility Criteria
Inclusion Criteria
- •Males, Generally healthy
- •Age 18-40 years old
- •BMI 18 - 25 kg/m2
- •No medication use that interfere with the aims of the study
- •Stable level of daily physical activity or training for at least 6 months
Exclusion Criteria
- •Regular smokers, drug abuse
- •Participation in other studies
- •Weight gain/loss \> 3 kg in the last 6 months
- •Contraindications for MRS scans:
- •Vegetarian eating behavior
Outcomes
Primary Outcomes
Skeletal muscle PCr on-kinetic during exercise and carnitine acetyltransferase (CrAT) protein content/activity
Time Frame: MRS+Exercise test day 2
The correlation between skeletal muscle PCr on-kinetic during one-legged exercise and CrAT protein activity will be determined.
Secondary Outcomes
- Skeletal muscle acetylcarnitine recovery after one-legged exercise and carnitine acetyltransferase (CrAT) protein content/activity(MRS+Exercise test day 2)
- Skeletal muscle oxygen availability(MRS test day 1)
- Skeletal muscle CrAT protein activity, from muscle biopsy, and PCr breakdown rate during one-legged exercise(MRS+Exercise test day 1)
- Skeletal muscle acetylcarnitine formation capacity upon exercise and total carnitine availability in skeletal muscle(MRS+Exercise test day 2)
- Skeletal muscle mitochondrial function in vitro(MRS+Exercise test day 2)