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Effect of Probiotics on Cardiometabolic Health

Not Applicable
Completed
Conditions
Cardiometabolic Risk Factors
Interventions
Dietary Supplement: Placebo control
Dietary Supplement: Probiotics
Registration Number
NCT05005754
Lead Sponsor
Sun Yat-sen University
Brief Summary

Atherosclerosis is a major risk factor for cardiovascular diseases. Recently, dysbiosis of gut microbiota has been reported to play an important role in the pathogenesis of atherosclerosis. Animal studies have demonstrated that administration of probiotics help delay the progression of atherosclerosis through several mechanisms. This trial aims to examine its protective effect in humans.

Detailed Description

Atherosclerosis is a major risk factor for cardiovascular diseases. Recently, dysbiosis of gut microbiota has been reported to play an important role in the pathogenesis of atherosclerosis. Animal studies have demonstrated that administration of probiotics help delay the progression of atherosclerosis through several mechanisms such as reduction in endotoxemia, and enhanced production of short-chain fatty acids.

However, whether administration of probiotics also has a protective effect in subjects with metabolic syndrome (MetS), who are at high risk for developing atherosclerosis, remain unknown.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
130
Inclusion Criteria
  • Local residents aged between 30-60 years old;
  • Stable weight (<5% weight change over last 3 months);
  • Central obesity defined as waist circumference ≥90 cm in males or ≥80 cm in females, Plus any two of the following four conditions i) Elevated triglycerides: serum triglycerides ≥150 mg/dL (1.7 mmol/L); ii) Reduced HDL cholesterol: serum HDL-c <40 mg/dL (1.03 mmol/L) in males or <50 mg/dL (1.29 mmol/L) in females; iii) Elevated blood pressure: systolic blood pressure ≥130 mmHg or diastolic blood pressure ≥85 mmHg; iv) Elevated fasting plasma glucose: fasting plasma glucose ≥100 mg/dL (5.6 mmol/L)
  • Absence of any systemic, cardiovascular diseases, as well as infections within the previous month;
  • Absence of any diet or medication that might interfere with metabolic homoeostasis and gut microbiota, especially antibiotics and probiotics 4 weeks before recruitment.
Exclusion Criteria
  • Acute illness or current evidence of acute or chronic inflammatory of infective diseases;
  • Participation in regular diet program more than 2 times per week in the latest 3 months prior to recruitment;
  • Mental illness rendering them unable to understand the nature, scope, and possible consequences of the study.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Placebo ControlPlacebo controlSubjects are instructed to take one capsule of placebo daily for a total of 3 months
ProbioticsProbioticsSubjects are instructed to take one capsule of probiotics daily for a total of 3 months
Primary Outcome Measures
NameTimeMethod
Flow-mediated slowing (FMS)from baseline to 12 weeks after intervention

FMS is a simple test for endothelial functions measured by Vicorder. In patients whose endothelial function or flow-mediated vasodilation are compromised, the FMS is substantially reduced.

Secondary Outcome Measures
NameTimeMethod
Insulin sensitivityfrom baseline to 12 weeks after intervention

alterations of HOMA-IR index

microbial metabolitesfrom baseline to 12 weeks after intervention

alterations of microbial metabolites evaluated by High performance liquid chromatography-mass spectrometry

gut microbiotafrom baseline to 12 weeks after intervention

Alterations of the composition of gut microbiota evaluated by metagenomics

Metabolic Syndrome Severity Z Score (MetZ score)from baseline to 12 weeks after intervention

MetZ score is an weighted score of the five traditional components of Metabolic syndrome (waist, triglyceride, HDL cholesterol, SBP and fasting glucose). It's used to evaluate the severity of metabolic disorder, and a higher score indicates a more severe status.

Lipid profilesfrom baseline to 12 weeks after intervention

alterations of blood lipids including total cholesterol, triglyceride, LDL cholesterol and HDL cholesterol determined by automatic biochemical analyser

Blood pressurefrom baseline to 12 weeks after intervention

alterations of blood pressure

Trial Locations

Locations (2)

Sun Yat-Sen University

🇨🇳

Guangzhou, Guangdong, China

Department of Nutrition and Food Hygiene

🇨🇳

Guangzhou, Guangdong, China

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