Swedish Iodine in Pregnancy and Development in Children (SWIDDICH) Study
- Conditions
- Thyroid Gland; NodeCognitive Developmental DelayPregnancy
- Interventions
- Dietary Supplement: IodineDietary Supplement: Placebo
- Registration Number
- NCT02378246
- Lead Sponsor
- Göteborg University
- Brief Summary
THE ULTIMATE GOAL of this project is to answer the question "In MILD IODINE DEFICIENCY (ID), should a tablet with vitamins and minerals, including 150 μg iodine/day be administered to pregnant women with a normal diet, to attain a normal cognitive development of the fetus or is there no cognitive deficit from mild ID and no extra iodine is needed?". To answer this question, the investigators planned a randomized, placebo-controlled trial of micronutrient supplementation during pregnancy in Sweden (SWIDDICH) with the follow-up of childrens' COGNITIVE DEVELOPMENT at 18 months, 3.5, 7 and 14 years.
Iodine deficiency (ID) is associated with thyroid morbidity and, especially in children, with impaired cognitive development. Sweden introduced iodine fortification of table salt 1936 and mental retardation due to severe ID is eradicated. Is mild ID during pregnancy also eradicated? If not, is this of importance? A national study performed by the investigators in 2007 showed iodine sufficiency in general population, but there are no pregnancy data. Local studies have raised concerns for mild ID during pregnancy in Sweden and a trans-sectional national study is currently ongoing.
The burning question for Sweden and the whole world is: is mild ID during pregnancy of importance for the developing brain of the fetus? Two large observational studies have shown association between mild ID during pregnancy and lower verbal IQ or educational performance at school-aged children. The world needs a randomized placebo-controlled trial (RTC) comparing the cognitive outcome in children exposed to mild ID during fetal life with children exposed to normal iodine levels during fetal life.
Our HYPOTHESIS is that pregnant women in Sweden have mild ID and that children exposed to mild ID during fetal life have a lower cognitive development, compared to children to mothers taking daily tablet with vitamins and minerals, including 150 ug iodine during pregnancy.
The MAIN AIM of the SWIDDICH trial is to determine if children exposed to deficient micronutrition including mild iodine deficiency (ID) during fetal life achieve worse cognitive development compared to children exposed to normal iodine status reached by maternal iodine supplementation.
- Detailed Description
AIMS
* To determine if children exposed to deficient micronutrition including mild iodine deficiency (ID) during fetal life achieve worse cognitive development compared to children exposed to normal iodine status reached by maternal iodine supplementation.
* To investigate the interaction between iodine, selenium and iron, as components of the intervention.
* To compare urinary iodine concentration (UIC), thyroglobulin (Tg), free tetraiodothyronine (FT4) and thyroid stimulating hormone (TSH) in pregnant women on daily tablet with vitamins and minerals, including 150 µg iodine or placebo
* To compare milk iodine concentration (MIC) in colostrum, and TSH in the first 200 newborn children to pregnant women receiving daily tablet with vitamins and minerals, including 150 µg iodine or placebo
BACKGROUND Iodine deficiency increases the risk for hypothyroidism and goiter and during the pregnancy for abortion or cognitive and other abnormalities of the baby. According to the WHO recommendations, UIC during pregnancy should be 150-249 ug/L. In 2007 a national study showed iodine sufficiency in Swedish general population. It is debated whether or not iodine shall be given to pregnant women in iodine sufficient populations.
The research group started this randomized iodine intervention in pregnant women in 2012. The first hypothesis was that daily supplementation with a tablet with vitamins and minerals including 150 µg iodine is needed during pregnancy in Sweden in order to ensure normal iodine and thyroid hormone status in mothers and newborn children and that pregnant women in Sweden suffer from mild iodine deficiency (ID).
There is a substantial gap of knowledge with regards to whether mild ID during fetal life entails negative consequences on cognition. Two large observational studies have shown association between mild ID during pregnancy and lower IQ or educational performance in school-children. The only two randomized placebo-controlled trials (RCTs) studying mild ID in pregnancy were either too small for safe conclusions to be drawn ( Brucker-Davis et al, n=86), or did not manage to separate groups based on iodine levels (the MITCH study, Melse-Boonstra et al, n=839). The need for a RCT with sufficient sample size remains. This led the research team to form a second hypothesis within the trial: children exposed to deficient micronutrition including mild ID during fetal life have lower cognitive development compared to children to mothers taking a daily tablet with vitamins and minerals including150 ug iodine during pregnancy. The decision to expand the trial was made and the target number of participants to be recruited increased from 200 to 1275 in order to reach enough power to follow-up the childrens' cognitive development.
METHODS
Design
This is a prospective placebo-controlled trial of children whose mothers got iodine supplement 150 µg/day (a tablet with vitamins and minerals) or placebo (a multivitamin preparation without minerals) during pregnancy (week 10±2 until delivery). The target number participants during pregnancy is 1275 to enable sufficient power in the children follow-up (n=788). The main outcome is intelligence quotient (IQ) at 3.5 year of age. Cognitive development in children will be also assessed at 18 months, 7, and 14 years.
Pregnancy part
Pregnant women from more than ten maternity care centers in Sweden are randomized to daily receive a multivitamin tablet with minerals, including 150 µg iodine or placebo (multivitamin without iodine). The intervention starts at pregnancy week 8-12 and ends directly post-partum for the first 200 women and at pregnancy week 36±2weeks for the remaining participants. This time point may be adjusted to local circumstances, depending on when during the third trimester the routine visit at maternity care center takes place. Participants are included after the first visit by a midwife. Urine sample for UIC and U-creatinine, and blood sample for thyroid hormones, thyroid stimulating hormone (TSH), thyroglobuline (Tg) and thyroperoxidase antibodies (TPO-ab) are collected and a simple questionnaire is filled in at week 10±2 weeks of pregnancy and placebo/intervention is started. Selenium and iron are also measured. The same measurements are collected in week 25±1 week and week 36±2 weeks. Directly after delivery (within 5 days), MIC and UIC in the first 200 mother as well as UIC and TSH in their newborn child were collected and a simple questionnaire is filled in. Weight, length and APGAR in the child and pregnancy complications are registered. Blood is also frozen for future analyses and access to medical files is accepted.
In parallel 90 healthy female controls from Skaraborg area stratified for age and smoking habits are collected from a randomized sample attained by the Swedish Tax Agency. UIC, u-creatinine, FT4, TSH, Tg, TPO-ab, and samples to be frozen are collected and a questionnaire is filled in. Selenium and iron will be analyzed in blood sample from the first 200 pregnant women and from controls. The primary purpose for having a control population is to ascertain that the normal population in the area of Skaraborg is iodine sufficient.
Children follow-up
At 18 months ± 1 month psychomotor development is assessed by means of ASQ (Ages and Stages Questionnaire). At 3,5 years ± 2 months the IQ is measured (WPPSI-IV), behavior is assessed (CBCL questionnaire) and urine is collected for UIC measurement. At 7 years ± 3 months the following are assessed: IQ (WISC IV), motor development (Movement ABC), behavior (CBCL), symptoms related to attention deficit and hyperactivity disorder (ADHD, 5-15 Nordic questionnaire). Additionally, at 7 years ± 3 months urine is collected for UIC measurement, blood sample is taken for thyreoglobulin (Tg), thyroid hormones and deiodinases, and MRI of the brain is performed in a subsample. At 14 years ± 6 months all these, except for the Movement ABC, are repeated. On all occasions information on socio-economic status and other possible confounders are collected by means of questionnaires filled by parents and by children (14 years).
Recruitment & Eligibility
- Status
- RECRUITING
- Sex
- Female
- Target Recruitment
- 1275
Not provided
Not provided
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Tablet with vitamins and minerals, containing 150 ug iodine, 1 tablet daily Iodine Table of contents: Vitamin B2 1.4 mg, Vitamin B12 15 µg, Iron 12 mg, Zinc 12 mg, Iodine 150 µg, Selenium 50 µg, Calcium 250 mg Placebo: non-iodine containing multivitamin, 1 tablet daily Placebo Table of contents: Vitamin A 400 µg, Vitamin B1 1.4 mg, Vitamin B2 1.7 mg, Vitamin B6 1.8 mg, Vitamin B12 3 µg, Vitamin C 60 mg, Vitamin D 5 µg, Vitamin E 10 mg, Niacin 19 mg, Folic acid 200 µg
- Primary Outcome Measures
Name Time Method Intelligence quotient (IQ) at 3.5 years, WPPSI IV when the child is at the age of 3,5 years ± 2 months Wechsler Preschool and Primary Scale of Intelligence - Fourth Edition; IQ with sub-domains
- Secondary Outcome Measures
Name Time Method Free thyroxine concentration in serum, in the mother, 2nd trimester 26±2 weeks (pregnancy) Anti-TPO in the mother, 3rd trimester 36±2 weeks (pregnancy) Thyroid peroxidase antibody titer, plasma
B12 in the mother, 1st trimester 10±2 weeks (pregnancy) Vitamin B12, serum
Urinary iodine concentration (UIC) at 14 years when the child is at the age of 14 years ± 6 months Thyroid stimulating hormon concentration in serum, in the mother, 1st trimester 10±2 weeks (pregnancy) Urinary iodine concentration (UIC) in the newborn within 5 days after delivery ADHD-related problems at 14 years, FTF when the child is at the age of 14 years ± 6 months Nordic Questionnaire 5-15 (FTF) filled in by parents
Urinary iodine concentration (UIC) in the mother, 1st trimester 10±2 weeks (pregnancy) Psychomotor development at 18 months, ASQ-3 when the child is at the age of 18 months ± 1 month Ages \& Stages Questionnaire - Third Edition; a questionnaire filled in by parents
Intelligence quotient (IQ) at 14 years, WISC IV when the child is at the age of 14 years ± 6 months Wechsler Intelligence Scale for Children - Fourth Edition; IQ with sub-domains
Behavioral problems at 7 years, CBCL when the child is at the age of 7 years ± 3 months Child Behavior Checklist questionnaire filled in by parents
Free thyroxine concentration in serum, in the mother, 1st trimester 10±2 weeks (pregnancy) Urinary iodine concentration (UIC) in the mother, 2nd trimester 26±2 weeks (pregnancy) Behavioral problems at 3.5 years, CBCL when the child is at the age of 3,5 years ± 2 months Child Behavior Checklist questionnaire filled in by parents
Motor skills at 7 years, Movement ABC when the child is at the age of 7 years ± 3 months Movement ABC test performed by a physiotherapist
Brain development at 7 years, MRI when the child is at the age of 14 years ± 6 months Magnetic Resonance Imaging; manual and automatic volumetry
Urinary iodine concentration (UIC) in the mother, 3rd trimester 36±2 weeks (pregnancy) Thyreoglobulin in serum, in the mother, 2nd trimester 26±2 weeks (pregnancy) Behavioral problems at 14 years, CBCL when the child is at the age of 14 years ± 6 months Child Behavior Checklist questionnaire filled in by parents
Thyroid stimulating hormon concentration in serum, in the mother, 2nd trimester 26±2 weeks (pregnancy) Thyroid stimulating hormon concentration in serum, in the mother, 3rd trimester 36±2 weeks (pregnancy) Selenium in the mother, 1st trimester 10±2 weeks (pregnancy) Selenium, serum
Iron in the mother, 2nd trimester 26±2 weeks (pregnancy) Iron, serum
Intelligence quotient (IQ) at 7 years, WISC IV when the child is at the age of 7 years ± 3 months Wechsler Intelligence Scale for Children - Fourth Edition; IQ with sub-domains
ADHD-related problems at 7 years, FTF when the child is at the age of 7 years ± 3 months Nordic Questionnaire 5-15 (FTF) filled in by parents
Thyreoglobulin concentration in serum, in the mother, 1st trimester 10±2 weeks (pregnancy) Thyreoglobulin in serum, in the mother, 3rd trimester 36±2 weeks (pregnancy) Free thyroxine concentration in serum, in the mother, 3rd trimester 36±2 weeks (pregnancy) Free thyroxine concentration, serum
Anti-TPO in the mother, 1st trimester 10±2 weeks (pregnancy) Thyroid peroxidase antibody titer, plasma
Anti-TPO in the mother, 2nd trimester 26±2 weeks (pregnancy) Thyroid peroxidase antibody titer, plasma
B12 in the mother, 2nd trimester 26±2 weeks (pregnancy) Vitamin B12, serum
Selenium in the mother, 2nd trimester 26±2 weeks (pregnancy) Selenium, serum
Milk iodine concentration (MIC) in colostrum within 5 days after delivery TSH at 14 years when the child is at the age of 14 years ± 6 months Thyroid stimulating hormone, dry blood spot (DBS)
TG at 7 years when the child is at the age of 7 years ± 3 months Thyreoglobulin, dry blood spot (DBS)
Deiodinases at 7 years when the child is at the age of 7 years ± 3 months Dry blood spot (DBS)
B12 in the mother, 3rd trimester 36±2 weeks (pregnancy) Vitamin B12, serum
Selenium in the mother, 3rd trimester 36±2 weeks (pregnancy) Selenium, serum
Iron in the mother, 3rd trimester 36±2 weeks (pregnancy) Iron, serum
Urinary iodine concentration (UIC) at 3.5 years when the child is at the age of 3,5 years ± 2 months Urinary iodine concentration (UIC) at 7 years when the child is at the age of 7 years ± 3 months Thyroid stimulating hormone, dry blood spot (DBS), in the newborn within 5 days after delivery TSH at 7 years when the child is at the age of 7 years ± 3 months Thyroid stimulating hormone, dry blood spot (DBS)
FT4 at 7 years when the child is at the age of 7 years ± 3 months Free thyroxine, dry blood spot (DBS)
Iron in the mother, 1st trimester 10±2 weeks (pregnancy) Iron, serum
Urinary iodine concentration (UIC) in the mother within 5 days after delivery TG at 14 years when the child is at the age of 14 years ± 6 months Thyreoglobulin, dry blood spot (DBS)
FT4 at 14 years when the child is at the age of 14 years ± 6 months Free thyroxine, dry blood spot (DBS)
Deiodinases at 14 years when the child is at the age of 14 years ± 6 months Dry blood spot (DBS)
Trial Locations
- Locations (3)
Linköping University Hospital
🇸🇪Linkoping, Sweden
Umeå Universitet, Department of Clinical Sciences
🇸🇪Umea, Västerbotten, Sweden
Sahlgrenska University Hospital
🇸🇪Gothenburg, Sweden