Detection and Pathogenesis of Novel Protein F
- Conditions
- Cardiac DiseaseMultiple Myeloma
- Registration Number
- NCT04338386
- Lead Sponsor
- Wuhan Asia Heart Hospital
- Brief Summary
From December 6, 2019 to March 23, 2020, the research group of Qingkun Fan found a novel protein(temporarily named protein F) in heparin anticoagulant plasma of three patients with heart disease. One patient was diagnosed with multiple myeloma.However, protein F cannot be detected by serum protein electrophoresis. Preliminary studies have shown that this novel protein F have an obvious absorption peak at about 600nm. Placed at 2-8 degrees for 7 days, protein F will be isolated from heparin plasma. To the naked eye, protein F appear to be transparent jelly between the red blood cells and the plasma. The specific protein F, how it is produced, how it causes disease are still unknown. This study will explore how to detect protein F and how it is produced.
- Detailed Description
Protein F screening was performed on anticoagulant blood plasma with heparin Lithium or blood serum by in-house turbidimetric method in Automatic biochemical analyzer.
All patients were followed-up for 3 years by telephones, routine appointment out-patient clinic or returning visit.
Recruitment & Eligibility
- Status
- NOT_YET_RECRUITING
- Sex
- All
- Target Recruitment
- 100
- Testing total protein and albumin
- Testing routine blood test
- high triglycerides (TG>5.00 mmol/L)
- hyperbilirubinemia (TBIL>170 mmol/L)
- fat emulsion used in past 3 days
- patients with hemolytic disease
- pregnant and lactating women
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Concentration of plasma protein F content six month the protein F content was measured by turbidimetric assay in automatic biochemical analyzer
- Secondary Outcome Measures
Name Time Method Number of Participants with Multiple myeloma 3 years Develop or be diagnosed with multiple myeloma