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Convalescent Plasma Therapy for Hospitalized Patients With COVID-19

Phase 2
Completed
Conditions
Coronavirus Infections
Convalescent Plasma
Respiratory Tract Diseases
COVID-19
SARS-CoV-2
Pneumonia
Interventions
Drug: Standard of care
Biological: Convalescent plasma
Registration Number
NCT05077930
Lead Sponsor
Tânia Portella Costa
Brief Summary

Plasma from donors who have recovered from coronavirus disease 2019 (COVID-19) contain antibodies to SARS-CoV-2 and may be a potential therapy for hospitalized patients with COVID-19. The efficacy of high-titer convalescent plasma for COVID-19, however, still unclear. The present study aims to evaluate the efficacy and safety of using convalescent plasma for treating hospitalized patients with COVID-19.

Detailed Description

This is an open-label, randomized controlled trial aimed to evaluate the efficacy and safety of using convalescent plasma for treating hospitalized patients with COVID-19. Participants must be adult hospitalized patients with a confirmed diagnosis of COVID-19 and time Between symptom onset and inclusion ≤ 7 days. Two hundred participants will be randomized in a 1:1 ratio to receive either 200-400 mL of high-titer COVID-19 convalescent plasma or standard care. The primary endpoint is the proportion of patients with clinical improvement at day 14 following randomization, defined by an increase of two points in the 7-point ordinal scale based on that recommended by the World Health Organization. Safety will be daily assessed by monitoring the occurrence of adverse effects and reactions to convalescent plasma transfusion. Study visits will occur on Day 1, Day 3, Day 7, and Day 14 or until hospital discharge, whichever comes first.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
38
Inclusion Criteria
  • Hospitalized patients aged ≥18 years.
  • Confirmed diagnosis of COVID-19 by RT-PCR or antigen test in respiratory samples.
  • Time between symptom onset and inclusion ≤ 7 days.
  • Enrolled within 5 days of hospitalization.
  • Sign the consent form.
Exclusion Criteria
  • Contraindication to transfusion due to inability to tolerate additional fluid, such as due to decompensated congestive heart failure.
  • History of previous severe allergic reactions to transfused blood products.
  • Limiting comorbidity for administering the therapies provided for in this protocol in the opinion of the investigator.
  • Not currently enrolled another interventional clinical trial of COVID-19 treatment.
  • Critically ill patient with COVID-19 being treated in intensive care.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Standard of careStandard of careStandard of care treatment according to the institutional protocol.
Convalescent PlasmaConvalescent plasmaThe investigational product is anti-SARS-CoV-2 convalescent plasma obtained from former patients identified as having recovered from COVID-19 and obtained by Centro de Hematologia e Hemoterapia do Paraná - Hemepar following national blood donation guidelines and Brazilian Health Regulatory Agency (ANVISA) criteria. Potential donors will be screened using an anti-SARS-CoV-2 serologic assay and antibody levels will be determined. Participants will receive the standard of care treatment and a single unit of convalescent plasma (volume=200 mL or 400 mL).
Primary Outcome Measures
NameTimeMethod
Clinical status on a 7-point ordinal scaleFrom randomization to end of study at Day 14

Patients' clinical status over time assessed by a 7-point ordinal scale from World Health Organization (WHO). Lower scores are seen with better clinical outcomes. The scale categories are as follows: (1), not hospitalized with resumption of normal activities; (2), not hospitalized, but unable to resume normal activities; (3), hospitalized, not requiring supplemental oxygen; (4), hospitalized, requiring supplemental oxygen; (5), hospitalized, requiring high-flow oxygen therapy or noninvasive mechanical ventilation; (6), hospitalized, requiring ECMO (extracorporeal membrane oxygenation), IMV (intermittent mandatory ventilation), or both; (7), death.

Proportion of patients with clinical improvement, defined by an increase of two points in the ordinal scale of seven WHO categories.

Secondary Outcome Measures
NameTimeMethod
Length of stay in intensive careDay 1, Day 3, Day 7, and Day 14 after randomization
Ventilator free daysDay 1, Day 3, Day 7, and Day 14 after randomization
Respiratory rateDay 1, Day 3, Day 7, and Day 14 after randomization
Percentage of participants at each clinical status on a 7-point ordinal scaleDay 1, Day 3, Day 7, and Day 14 after randomization

Measure of patients' clinical status using an ordinal scale for clinical improvement created by World Health Organization (WHO) and based on 7-point scale categories. Lower scores in this scale are seen with better clinical outcomes. The scale categories are as follows: (1), not hospitalized with resumption of normal activities; (2), not hospitalized, but unable to resume normal activities; (3), hospitalized, not requiring supplemental oxygen; (4), hospitalized, requiring supplemental oxygen; (5), hospitalized, requiring high-flow oxygen therapy or noninvasive mechanical ventilation; (6), hospitalized, requiring ECMO, IMV, or both; (7), death.

Oxygen saturationDay 1, Day 3, Day 7, and Day 14 after randomization
Time until independence from oxygen therapy in daysDay 1, Day 3, Day 7, and Day 14 after randomization
In patients who needed mechanical ventilation, time to initiate mechanical ventilation (calculated in days, from entry into the protocol until orotracheal intubation)Day 1, Day 3, Day 7, and Day 14 after randomization
Percentage of participants who develop serious adverse events and adverse events considered as definitely or probably associated with plasma transfusionDaily, until Day 14 after randomization

Adverse events (worsening anemia, urticaria, skin rash, transfusion-associated circulatory overload, and others) assessed during hospitalization.

The PaO2 / FiO2 ratio (for patients on mechanical mechanisms)Day 1, Day 3, Day 7, and Day 14 after randomization
Number and /or extension of affected lung areas on chest computed tomographyDay 1, Day 3, Day 7, and Day 14 after randomization
Length of hospital stayDay 1, Day 3, Day 7, and Day 14 after randomization
Rate of transfusion reactions to convalescent plasma infusionDaily, until Day 14 after randomization
Prevalence of oxygen-intake methodsDay 1, Day 3, Day 7, and Day 14 after randomization

Percentage of participants using oxygen by mask or nasal prongs, oxygen by non-invasive ventilation or high flow, intubation \& mechanical ventilation and ECMO.

Trial Locations

Locations (1)

Maternidade e Cirurgia Nossa Senhora do Rocio/ SA - Hospital do Rocio

🇧🇷

Campo Largo, Paraná, Brazil

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