MRS to Determine Neuroinflammation and Oxidative Stress in MPS I
- Conditions
- Mucopolysaccharidosis Type I
- Registration Number
- NCT03576729
- Lead Sponsor
- University of Minnesota
- Brief Summary
Neuroinflammation and oxidative stress have been shown to be present in persons with mucopolysaccharidosis type I (MPS I), but their effect on disease severity and disease progression is unknown. The investigator intends to employ brain magnetic resonance spectroscopy (MRS), a non-invasive technique, along with analysis of neuroinflammation and oxidative stress biomarkers in the blood, to measure and determine the level of oxidative stress and neuroinflammation, and their impact on clinical variability in MPS I patients.
- Detailed Description
Persons with MPS I have a wide range of clinical manifestations including central nervous system (CNS) impairment. The role of neuroinflammation and oxidative stress is one avenue of investigation which may clarify the broad neurological impairment in MPS I. Finding biomarkers that accurately describe the underlying and ongoing brain pathology is a key not only to understanding the disease, but also to understanding the possibility of new therapeutic approaches for MPS I patients.
The investigator will compare patients with Hurler syndrome, and Hurler-Scheie or Scheie syndrome, with healthy controls. There will be 10 participants in each group, resulting in a total of 30 participants. Within the Hurler-Scheie or Scheie syndrome group, the investigator will examine the association of clinical severity with the proposed measures. These findings might help determine whether hematopoietic cell transplantation (HCT), which is the treatment for Hurler syndrome patients, results in decreased oxidative stress and neuroinflammation as compared to Hurler-Scheie or Scheie syndrome patients, who are treated by enzyme replacement therapy (ERT). Additionally, these findings might help determine whether therapies directed at reducing neuroinflammation and oxidative stress in MPS I could enhance neurological outcomes.
Study hypothesis: neuroinflammation and oxidative stress are present in MPS I subjects and are reflective of disease severity.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 30
MPS I participants must meet the following:
- Diagnosis of Hurler syndrome, OR Hurler-Scheie syndrome, OR Scheie syndrome
- 6 years of age or older at time of screening
Healthy control participants must meet all of the following:
- Absence of neurological disorder
- 6 years of age or older at time of screening
Persons who have any of the following will not be enrolled in this study:
- Any surgically implanted pacemaker
- Any indwelling electronic device, including programmable shunts
- Orthodontic braces, unless non-metallic
- Other implanted metal in the body other than titanium
- An inability or unwillingness to complete an MRI/MRS because of low cognitive function or behavioral dysregulation
- Pregnancy
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Brain Magnetic Resonance Imaging/Magnetic Resonance Spectroscopy (MRI/MRS) 1 day -Single encounter during an appointment which is set at time of study enrollment. In a single session, each participant will undergo unsedated brain magnetic resonance imaging/magnetic resonance spectroscopy (MRI/MRS) to determine the presence and extent of any brain neuroinflammation. These data will be acquired on the 7-Tesla Siemens Prisma scanner at the Center for Magnetic Resonance Research (CMRR) at the University of Minnesota in Minneapolis.
- Secondary Outcome Measures
Name Time Method Presence and Level of Interleukin 1 beta (IL1β) 1 day -Single blood draw performed at the same time as the single neuroimaging encounter. The presence of interleukin 1 beta (IL1β) will be determined. If present, the level of this inflammatory biomarker will be determined.
Presence and Level of Interleukin 2 (IL2) 1 day -Single blood draw performed at the same time as the single neuroimaging encounter. The presence of interleukin 2 (IL2) will be determined. If present, the level of this inflammatory biomarker will be determined.
Presence and Level of Regulated and Normal T cell Expressed and Secreted (RANTES) 1 day -Single blood draw performed at the same time as the single neuroimaging encounter. The presence of 'regulated and normal T cell expressed and secreted' (referred to as RANTES), alternatively also known as chemokine (C-C motif) ligand 5, or CCL5, will be determined. If present, the level of this inflammatory biomarker will be determined.
Presence and Level of Tumor Necrosis Factor Alpha (TNF-α) 1 day -Single blood draw performed at the same time as the single neuroimaging encounter. The presence of tumor necrosis factor alpha (TNF-α) will be determined. If present, the level of this inflammatory biomarker will be determined.
Presence and Level of Neuroinflammatory Biomarker MIP-1alpha 1 day -Single blood draw performed at the same time as the single neuroimaging encounter. The presence of macrophage inflammatory protein (MIP)-1α (MIP-1alpha) will be determined; and if present, the level of this inflammatory biomarker will be determined.
Presence and Level of Interferon-gamma (IFN-γ) 1 day -Single blood draw performed at the same time as the single neuroimaging encounter. The presence of interferon-gamma (IFN-γ) will be determined. If present, the level of this autoinflammatory biomarker will be determined.
Presence and Levels of Thiobarbituric Acid Reactive Substances (TBARS) 1 day -Single blood draw performed at the same time as the single neuroimaging encounter. The presence of thiobarbituric acid reactive substances (TBARS), which are biomarkers of the damage produced by oxidative stress, will be determined. If present, the levels of these biomarkers will be determined.
Presence and Level of Interleukin 8 (IL8) 1 day -Single blood draw performed at the same time as the single neuroimaging encounter. The presence of interleukin 8 (IL8), alternatively referred to as chemokine (C-X-C motif) ligand 8, or CXCL8, will be determined. If present, the level of this inflammatory biomarker will be determined.
Determination of Blood Glutathione Redox Ratio 1 day -Single blood draw performed at the same time as the single neuroimaging encounter. The blood glutathione redox ratio will be determined.
Presence and Level of Superoxide Dismutase (SOD) 1 day -Single blood draw performed at the same time as the single neuroimaging encounter. The presence of superoxide dismutase (SOD) will be determined. If present, the level of this antioxidant will be determined.
Presence and Level of Total Glutathione 1 day -Single blood draw performed at the same time as the single neuroimaging encounter. The presence of total glutathione will be determined. If present, the level of this antioxidant will be determined.
Presence and Level of 8-isoprostane 1 day -Single blood draw performed at the same time as the single neuroimaging encounter. The presence of 8-isoprostane will be determined. If present, the level of this inflammatory biomarker will be determined.
Presence and Level of 4-hydroxynonenal (4-HNE) 1 day -Single blood draw performed at the same time as the single neuroimaging encounter. The presence of 4-hydroxynonenal (4-HNE) will be determined. If present, the level of this oxidative stress biomarker will be determined.
Presence and Level of Catalase 1 day -Single blood draw performed at the same time as the single neuroimaging encounter. The presence of catalase will be determined. If present, the level of this oxidative stress biomarker will be determined.
Trial Locations
- Locations (1)
University of Minnesota
🇺🇸Minneapolis, Minnesota, United States