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Fecal Microbiota Transplantation in Pediatric Ulcerative Colitis (UC)

Not Applicable
Recruiting
Conditions
Ulcerative Colitis
Interventions
Other: Fecal Microbiota Transplantation
Registration Number
NCT05679622
Lead Sponsor
Biao Zou
Brief Summary

This study included two topics: one was to test the efficacy and safety of fecal microbiota transplants plus partial enteral nutrition (PEN) in refractory pediatric UC where conventional therapy has failed, and the other was to explore the efficacy and safety of FMT plus PEN as first-line therapy for pediatric active UC

Detailed Description

Recent studies have suggested that gut imbalance and deregulation of immunological responses plays a pivotal role in the disease development of UC, and that FMT could be a useful treatment. In the refractory ulcerative colitis group, our study is aims to explore FMT plus PEN in the treatment of refractory pediatric UC. In the induction stage of UC, standard therapy remained unchanged, FMT and PEN treatment are added, and the investigators hope the withdrawal of conventional drug therapy was gradually reduced. Refractory UC is defined as refractory to standard therapy (e.g., steroids, immunomodulators, cyclosporine, tacrolimus, or anti-TNF agents).

As a first-line treatment group for UC, our study is aims to explore FMT plus PEN as a first-line treatment for active UC in children. participants treated with FMT coupled with PEN are defined as the FMT group, and those treated with PEN coupled with mesalazine served as the PEN group.

FMT treatment is given for at least one course of FMT treatment. If repeated FMTs are received, it is usually at a 2 month interval.

All the participants received PEN (80% of total calories as a polymeric diet, Peptamen, Nestle, Vevey, and Switzerland) intervention to help induce and maintain clinical remission.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
30
Inclusion Criteria

age of older than 2 years and younger than 16 years with no genetic diseases; as a first-line treatment group for UC, newly diagnosed with mild-to-moderate UC (defined by the PUCAI of >10 and≤64); In the refractory ulcerative colitis group, all refractory pediatric with mild-to-moderate UC (defined by the PUCAI of >10 and≤64) defined by children who failed conventional treatment (hormone, immunosuppressant, biologics); agree to received regularly colonoscopy

Exclusion Criteria

Children who were treated by PEN (80%) less than 8 weeks; As a first-line treatment group for UC, patients who were treated with corticosteroids, methotrexate, thiopurines, and anti-TNF agents as their first-line treatment; Known contraindication to all FMT infusion method such as nasoduodenal tube insertion, oesophago-gastro-duodenoscopy (OGD), enteroscopy, colonoscopy, enema and Fecal capsule; Unwilling to give informed consent/ assent

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
FMT groupFecal Microbiota TransplantationIn the refractory ulcerative colitis group, our study is aims to explore repeated and multiple FMTs plus PEN in the treatment of refractory pediatric UC;
Primary Outcome Measures
NameTimeMethod
clinical response8-12 weeks after FMT

reduction in the Pediatric Ulcerative Colitis Activity Index (PUCAI) ≥30% from baseline

clinical remission8-12 weeks after FMT

Clinical remission defined as a PUCAI \<10

safety of FMT8-12 weeks after FMT

All possible adverse events: fever, abdominal pain, infectious diseases and others.

Secondary Outcome Measures
NameTimeMethod
Number of patients requiring escalation of medical therapies8-12 weeks after FMT

Number of patients requiring escalation of medical therapies based on clinical relapse. Clinical relapse is defined by requiring additional medical therapy.

Number of patients with endoscopic remission8-12 weeks after FMT

Number of patients with endoscopic remission as defined by a PUCAI score of 0

Fecal calprotectin level8-12 weeks after FMT

Mean change of Fecal calprotectin levels

C-reactive protein levels8-12 weeks after FMT

Mean change of C-reactive protein levels

erythrocyte sedimentation rate (ESR) level8-12 weeks after FMT

Mean change of erythrocyte sedimentation rate (ESR)

The number of stools or bloody stools8-12 weeks after FMT

Improvement in the number of stools or bloody stools

gut microbialbefore treatment and 4 weeks after treatment

Fecal 16S RNA or macrogene sequencing was performed. Fecal samples were obtained from donor and recipient. The fecal samples and isolated microbiota samples were frozen immediately and underwent DNA extraction using standard methods.

Trial Locations

Locations (1)

Tongji Hospital

🇨🇳

Wuhan, China

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