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Phase II Clinical Trial of Recombinant SARS-CoV-2 Spike Protein Vaccine (CHO Cell) for the Prevention of COVID-19

Phase 2
Active, not recruiting
Conditions
COVID-19
Interventions
Biological: 202-CoV standard dose
Biological: 202-CoV low adjuvant dose
Biological: 202-CoV low antigen dose
Biological: Placebo
Registration Number
NCT04990544
Lead Sponsor
Shanghai Zerun Biotechnology Co.,Ltd
Brief Summary

The purpose of this double-blind, randomized, controlled study is to assess immunogenicity and safety of 202-CoV at multiple dose levels, administered as 2 injections (i.m) at 28 days apart in adult subjects 18 years of age and above.

Detailed Description

Not available

Recruitment & Eligibility

Status
ACTIVE_NOT_RECRUITING
Sex
All
Target Recruitment
528
Inclusion Criteria
  • Healthy individuals aged 18-59 years as well as 60 years and above who can provide legal identification (males and females are both required).
  • Willing to participate in the study with informed consent prior to screening
  • Negative in SARS-CoV-2 IgG and IgM test at screening.
  • Women of childbearing potential must be using effective method of birth control for 14 days prior to the enrollment of the study and must agree to continue such precautions during the study until 30 days after the second dose of the study vaccine/placebo.
  • Male subjects must agree to employ acceptable contraception from the day of first dose of the study vaccine/placebo until 30 days after the second dose of the study vaccine/placebo.
Exclusion Criteria
  • Confirmed or asymptomatic COVID-19 cases or SARS-CoV-2 infection(had positive in SARS-CoV-2 nucleic acid test or serological test).
  • Had a history of traveling or residence in domestic area of high pandemic risk, overseas or epidemic areas, or had a history of contact with confirmed, asymptomatic or suspected COVID-19 cases within the past 14 days;
  • History of SARS;
  • Received SARS-CoV-2 vaccines for emergency use or approved SARS-CoV-2 vaccines;
  • Individuals involving a clinical study within 6 months prior to the screening visit; or planning to participate in another clinical study during study period.
  • Individual's systolic blood pressure ≥ 150mmHg and/or diastolic blood pressure ≥ 100mmHg at screening visit
  • Axillary temperature >=37.3℃ prior to vaccination
  • Individuals in other acute diseases, or in the acute phase of chronic diseases within 3 days prior to the signing of the informed consent form.
  • Received immunoglobulin and/or blood product 3 months prior to the first vaccination.
  • Presence of uncontrolled chronic pulmonary, cardiovascular, renal, hepatic, neurologic, hematologic or metabolic (including diabetes mellitus) disorders, which would include the potential subject in a high-risk category for SARS-CoV-2 infection and/or its complications as judged by the investigator.
  • Individuals with a history of severe allergic reaction (throat swelling, difficult in breath, dyspnea, or shock).
  • Individuals who have a history of severe adverse reaction associated with a vaccine or severe allergic reaction [e.g., anaphylaxis to any component of the study vaccines (S protein, Aluminum hydroxide, CpG adjuvant).
  • Any autoimmune or immunodeficiency disease/condition [e.g. human immunodeficiency virus (HIV) infection, Systemic lupus erythematosus (SLE)]
  • Received immunoglobulin, blood-derived products within 3 months prior to the first study vaccination.
  • Abnormal coagulation function (such as coagulation factor deficiency, coagulation disease, platelet abnormality) obvious bruises or coagulopathy.
  • Pregnant women or breastfeeding women.
  • According to the judgment of the investigator, subject has or had any other symptoms, medical history and other factors that are not suitable for participating in this clinical trial.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Adult Group 2c202-CoV standard doseAdult healthy subjects (18 to 59 years of age, inclusive) receive 202-CoV standard dose at Day 0 and Day 28.
Elderly Group 2f202-CoV standard doseAdult healthy subjects (60 years of age and above) receive 202-CoV standard dose at Day 0 and Day 28
Elderly Group 2d202-CoV low adjuvant doseAdult healthy subjects (60 years of age and above) receive 202-CoV low adjuvant dose at Day 0 and Day 28
Adult Group 2b202-CoV low antigen doseAdult healthy subjects (18 to 59 years of age, inclusive) receive 202-CoV low antigen dose at Day 0 and Day 28
Elderly Group 2e202-CoV low antigen doseAdult healthy subjects (60 years of age and above) receive 202-CoV low antigen dose at Day 0 and Day 28
Adult Group 2a202-CoV low adjuvant doseAdult healthy subjects (18 to 59 years of age, inclusive) receive 202-CoV low adjuvant dose at Day 0 and Day 28
Adult PlaceboPlaceboAdult healthy subjects (18 to 59 years of age, inclusive) receive 2 doses of placebo (saline) at Day 0 and Day 28
Elderly PlaceboPlaceboAdult healthy subjects (60 years of age and above) receive 2 doses of placebo (saline) at Day 0 and Day 28
Primary Outcome Measures
NameTimeMethod
Seroconversion rate (SCR) of SARS-CoV-2 neutralising antibodies56 days

Neutralizing antibody activity as detected by neutralization assay expressed as seroconversion rate at multiple time points through Day 56

Geometric mean titer (GMT) of serum IgG antibodies56 days

Serum IgG antibody levels specific for the SARS-CoV-2 S protein antigen as detected by ELISA expressed as GMTs at multiple time points through Day 56

Geometric mean fold rise (GMFR) of SARS-CoV-2 neutralising antibodies56 days

GMFR in SARS-CoV-2 serum neutralizing titers from before vaccination to each subsequent time point

Geometric mean fold rise (GMFR) of serum IgG antibodies56 days

GMFR of serum IgG antibodies from before vaccination to each subsequent time point

Geometric mean titer (GMT) of SARS-CoV-2 neutralising antibodies56 days

Neutralizing antibody activity as detected by neutralization assay expressed as GMTs at multiple time points through Day 56

Seroconversion rate (SCR) of serum IgG antibodies56 days

Serum IgG antibody levels specific for the SARS-CoV-2 S protein antigen as detected by ELISA expressed as seroconversion rate at multiple time points through Day 56

Secondary Outcome Measures
NameTimeMethod
Percentage of participants reporting adverse events (AEs)From dose 1 through 28 days after the last dose

Percentage of participants with solicited AEs (local, systemic) for 28 days following each primary vaccination (Days 0, 28) by intensity, relevance.

Percentage of participants reporting solicited AEsFor 7 days after dose 1 and dose 2

Percentage of participants with solicited AEs (local, systemic) for 7 days following each primary vaccination (Days 0, 28) by intensity, relevance.

Percentage of participants reporting adverse events of special interest (AESIs)From dose 1 through 12 months after the last dose

Percentage of participants with AESI from dose 1 through 12month after last dose vaccination

Percentage of participants reporting unsolicited AEsFrom dose 1 through 28 days after the last dose

Percentage of participants with unsolicited AEs for 28 days following each vaccination

Percentage of participants reporting serious adverse events (SAEs)From dose 1 through 12 months after the last dose

Percentage of participants with SAEs from dose 1 through 12month after last dose vaccination

Trial Locations

Locations (1)

Xiangcheng Center for Disease Control and Prevention

🇨🇳

Xuchang, China

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