Quantitation of Bacteria Associated With Severe Periodontitis by Real -Time PCR Following Application of Diode Laser

Phase 2

Completed

• Conditions: Periodontal Pocket
• Interventions: Device: medency diode laser; Drug: spirazole forte

• Registration Number: NCT05222737
• Lead Sponsor: Alexandria University

Brief Summary

1. Primary objective To measure the gingival crevicular levels of A.actinomycetemcomitans and P.gingivalis after intra-pocket application of diode laser in severe periodontitis (stage 3 grade C) patients.

2. Secondary objective To compare the levels of the same microorganisms as in cases of diode laser therapy to those after conventional treatment with systemic antibiotic administration in severe periodontitis (stage 3 grade C) patients.

3. to measure clinical parameters probing depth,attachment loss and mobility grade

Detailed Description

Not available

Study Timeline

• Study Start: 2021-01-01
• Primary Completion: 2021-09-01
• Study Completion: 2021-12-01
• Study First Submitted: 2022-01-25
• Study First Submitted QC: 2022-01-25
• Study First Posted: 2022-02-03
• Status Verified: 2022-07
• Last Update Submitted: 2022-07-01
• Last Update Posted: 2022-07-06

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 50

Inclusion Criteria

1. Patients of both sexes having severe periodontitis (stage 3 grade C) with interdental clinical attachment loss (CAL) ≥ 5.
2. Patients' age between 15 and 35 years old with rapid bone loss showed by radiograph.
3. Systemically healthy patients.
4. Radiographic bone loss extending to middle or apical third of root.
5. Probing depth (PD) ≥ 6 mm.

Exclusion Criteria

1. Use of antibiotics during the last 3 months.
2. Pregnancy.
3. Any systemic condition that might affect the study.
4. Use of mouthwash containing antimicrobials during the previous 3 weeks.
5. Smoking.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
test group	medency diode laser	intra pocket diode laser application
control group	spirazole forte	antibiotic adminstration

Primary Outcome Measures

Name	Time	Method
Quantitation of Aggrecatibacter .actinomycetemcomitans and porphyromonas gingivalis bacteria	baseline	To measure the gingival crevicular levels of A.actinomycetemcomitans and P.gingivalis after intra-pocket application of diode laser in severe periodontitis(stage 3 grade C) patients

Secondary Outcome Measures

Name	Time	Method
comparing levels of bacteria between test and control groups	baseline	To compare the levels of the same microorganisms as in cases of diode laser therapy to those after conventional treatment with systemic antibiotic administration in severe periodontitis ( stage 3 grade C) patients.

Trial Locations

Locations (1)

Amira Hafez; 🇪🇬 Alexandria, Egypt