ocal and systemic antibody responses for maintaining gingival health
Completed
- Conditions
- gum inflammationperiodontitis10002252
- Registration Number
- NL-OMON41332
- Lead Sponsor
- Academisch Centrum Tandheelkunde Amsterdam (ACTA)
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 60
Inclusion Criteria
* >18 and < 60 years of age
* DPSI-score between 0 and 3- for healthy controls
* No bone loss visible on radiograph for healthy controls
* Bleeding on probing for periodontitis patients
* Periodontitis patients diagnosed with periodontitis according to definition by Tonetti & Claffey (2005, Journal of Clinical Periodontology) : proximal attachment loss of * 3 mm in * 2 non-adjacent teeth
* Otherwise healthy
* Informed written consent
Exclusion Criteria
* <18 and >60 years of age
* Used antibiotics in the last 3 months
* People receiving immunosuppressive therapy or taking other immunomodulating agents
* Having received periodontal treatment <2 years ago or in periodontal maintenance
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>From each patient, the relative frequencies of B- and T-cell subsets will be<br /><br>determined, both in tissue and blood. These parameters will be compared between<br /><br>patients and controls. Additionally, immunohistochemical analysis of paraffin<br /><br>embedded biopsies will be performed to assess overall morphology and cell types<br /><br>present. Finally, antibodies will be cloned from single sorted plasma cells,<br /><br>and these will be tested for their (auto-) reactivity. This will all be related<br /><br>to the bacterial composition in periodontitis. </p><br>
- Secondary Outcome Measures
Name Time Method <p>nvt</p><br>