Evaluation Of Lipid Peroxidation, Oxidative DNA Damage And Total Antioxidant Status In Patients With Periodontitis And Polycystic Ovary Syndrome: A Cross Sectional Study
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 88
- 试验地点
- 1
- 主要终点
- Plaque Index (PI)
研究概览
简要总结
Background: The aim of this study was to investigate the levels of malondialdehyde (MDA), total antioxidant status (TAS), 8-hydroxy-2´- deoxyguanosine (8-OHdG) in blood serum and saliva samples in female patients with chronic periodontitis (CP) and to compared healthy individuals.
Materials and Methods: A total of 88 women were into four groups each which were consisted of twenty-two subjects. Groups were designed as periodontally and systemically healthy women (PCOS-CP-); periodontally healthy women with polycystic ovary syndrome (PCOS) (PCOS+CP-), systemically healthy women with CP (PCOS-,CP+) and women with PCOS and CP (PCOS+CP+). Serum and salivary samples were obtained, clinical periodontal parameters were recorded. MDA, TAS, 8-OHdG levels were measured as biochemically.
详细描述
RESULTS:
Salivary 8-OHdG levels in the PCOSCP and CP groups were statistically higher than those in both the PCOSPH and the PH groups (P < 0.05). There was no statistical difference between the PCOSCP, CP, and PCOSPH groups with regard to salivary MDA and TAS levels (P > 0.05). Highest serum 8-OHdG and MDA levels and lowest serum TAS levels were seen in the PCOSCP group (P < 0.05). Serum 8-OHdG and MDA levels in the PCOSPH group were higher than those in both systemically healthy groups (PH and CP) (P < 0.05). Salivary TAS levels were highest (P < 0.05) in the PH group. There was no statistical difference between the CP and PCOSPH groups, but serum TAS levels were lower than those in the PH group (P < 0.05).
CONCLUSIONS:
CP, which led to an increase in serum and salivary 8-OHdG and MDA levels and a decrease in serum TAS levels in patients with PCOS, contributed to increased OS. This effect was more prominent in serum levels than in salivary levels.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •Newly diagnosed non-obese PCOS patients
- •Never smokers
- •Had no history of systemic disease
- •BMI<25 kg/m2
- •Participants had ≥20 teeth present.
排除标准
- •Pregnancy
- •Lactation
- •Hemoglobin A1c (HbA1c) ≥ 6.5%
- •2-h oral glucose tolerance test (OGTT-2h) ≥200
- •Cushing syndrome, non-classic congenital adrenal hyperplasia, hyperprolactinemia, thyroid dysfunction, and androgen-secreting tumors
- •Any drug use within the past 6 months
- •Periodontal treatment within the past 6 months
结局指标
主要结局
Plaque Index (PI)
时间窗: appoximately 2 years
Silnes-Löe Periodondal İndex
8-OHdG levels (nanogram per mililiter(ng/mL))
时间窗: appoximately 2 years
by ELISA test
Gingival Index (GI)
时间窗: appoximately 2 years
Löe-Silness Periodontal Index
Bleeding on probing (BOP)
时间窗: appoximately 2 years
Gİngival Bleeding Index
Probing pocket depth (PD))
时间窗: appoximately 2 years
distance between free gingival margin and periodontal pocket base by usin periodontal probe
Clinical attachment level (CAL)
时间窗: Patients were seen one time
by measuring the distance between the cementoenamel junction and the periodontal pocket base
TAS levels (millimolar per liter (mmol /L))
时间窗: appoximately 2 years
by ELISA test
MDA levels (micromole per liter (µmol/L))
时间窗: appoximately 2 years
by ELISA test
次要结局
- numberer of individuals with polycystic ovary syndrome(appoximately 2 years)
- number of individuals with chronic periodontitis(appoximately 2 years)