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PreFem: "What Happens to the Vaginal Microbiota When a BV Infection is Treated With Metronidazole?"

Not Applicable
Completed
Conditions
Bacterial Vaginosis
Interventions
Other: Metronidazole
Registration Number
NCT03187457
Lead Sponsor
Danisco
Brief Summary

The study will assess vaginal microbiota levels before and after antibiotic treatment in females infected with Bacterial Vaginosis (BV).

Detailed Description

This method validation study will endeavour to obtain evidence for:

* Recruitment methodology effectiveness

* The usefulness of the "Canestest" test as a screening tool

* The time required for the vaginal microbiota (particularly Lactobacillus count) to return to normal after treatment of BV infection with metronidazole.

* The time required for the Nugent Score to return to normal after treatment of BV infection with metronidazole.

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
60
Inclusion Criteria
  • Signed informed consent
  • Female gender
  • Over 18 years of age
  • Have a high probability for compliance with and completion of the study
Exclusion Criteria
  • Hypersensitivity to metronidazole --only applies to Group 2.
  • Post-menopausal defined as at least 12 consecutive months without menstruation
  • Treatment of BV in last 4 weeks
  • Clinically significant menstrual irregularities
  • Suspected presence of STDs or other vaginal infection
  • Pregnancy
  • Breast feeding
  • Participation in other clinical studies which could influence genitourinary tract microbiota
  • Substance abuse
  • Investigator believes that the participant may be uncooperative and/or noncompliant and should therefore not participate in the study

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Treatment groupMetronidazoleMetronidazole 400 mg 3 times daily for 5 days for Bacterial Vaginosis (BV) infection
Primary Outcome Measures
NameTimeMethod
Change in vaginal microbiota assessed with sequencingBaseline, at day 8, and at day 15

Change in vaginal microbiota is assessed with sequencing at 3 time points (baseline, at day 8 and at day 15 time points post antibiotic treatment.)

Secondary Outcome Measures
NameTimeMethod
Vaginal microbiota/lactobacilli levels with Nugent scoringBaseline, at day 8, and at day 15

Nugent scoring is used for the evaluation of the vaginal microbiota at baseline, at 8 and 15 day time points post antibiotic treatment.

Diagnosis of BV with "Canestest"Baseline

"Canestest" will be used to diagnose BV infection at baseline.

Trial Locations

Locations (1)

CPS Research

🇬🇧

Glasgow, United Kingdom

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