PGS on Blastocysts From Vitrified and Fresh Oocytes

Completed

• Conditions: Increase the Number of Blastocyst to Biopsy for Preimplantation Genetic Testing

• Registration Number: NCT02820415
• Lead Sponsor: Unità di Medicina della Riproduzione, Italy

Brief Summary

Oocyte accumulation by vitrification is a valid strategy to increase the number of available embryos for biopsy and the number of viable euploid embryos to transfer after preimplantation genetic testing.

Detailed Description

In the present study, the investigators considered the hypothesis to accumulate vitrified oocytes with a view to increasing the number of oocytes for micro-injection and consequently the number of blastocyst to diagnose for patients with normal ovarian reserve and candidate for PGT. The partecipants were proposed to undergo several cycles of ovarian stimulation. In the first cycles, mature (metaphase II) oocytes were vitrified and consequently accumulated. In the last cycle, the freshly produced mature oocytes and the previously accumulated ones were micro-injected together with the same partner's semen sample. PGT was performed on blastocysts produced from the two sources of oocytes. The comprehensive chromosomal analysis of biopsied trophectoderm cells was performed using NGS technology. The number of available euploid blastocyst and the proportion of euploid/aneuploidy embryos from fresh and vitrified/warmed oocytes were compared. In conclusion the investigators evaluated the efficacy of oocyte accumulation by vitrification in increasing the number of available embryos for biopsy and the number of viable euploid embryos to transfer after PGT.

Study Timeline

• Study Start: 2014-06
• Primary Completion: 2015-06
• Study Completion: 2015-12
• Status Verified: 2016-06
• Study First Submitted: 2016-06-24
• Study First Submitted QC: 2016-06-30
• Last Update Submitted: 2016-06-30
• Study First Posted: 2016-07-01
• Last Update Posted: 2016-07-01

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 60

Inclusion Criteria

• infertile women
• normal ovarian reserve
• with repeated implantation failure or reccurent miscarriage
• undergoing ICSI and asking to know the state of health of the embryos prior to embryo transfer

Exclusion Criteria

Not provided

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
rate of euploidy in blastocyst from fresh and vitrified/warmed oocytes	1 year	from fresh and vitrified/warmed oocytes

Trial Locations

Locations (1)

Sandrine Chamayou; 🇮🇹 Sant'agata Li Battiati, Italy