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Early and late effects of 24 hours supplemental parenteral amino acids on whole-body protein turnover in critically ill patients.

Not Applicable
Completed
Conditions
critical illness
Diet and Nutrition - Other diet and nutrition disorders
Metabolic and Endocrine - Other metabolic disorders
Registration Number
ACTRN12615001314516
Lead Sponsor
Olav Rooyackers
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
Completed
Sex
All
Target Recruitment
10
Inclusion Criteria

Critically ill patients with expected stable nutrition for 30 hours.

Exclusion Criteria

Ongoing dialysis

Study & Design

Study Type
Interventional
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Whole-body protein balance.<br><br>Whole-body protein balance is calculated by the difference between whole-body protein synthesis and breakdown rates which are assessed by the infusion of stable isotope labelled phenylalanine and tyrosine, detection of its amounts in plasma of the patients by mass spectrometry and kinetic modelling.[Whole-body protein balance measured just before (baseline) and at 3 and 24 hours after start of the intervention.]
Secondary Outcome Measures
NameTimeMethod
Whole-body phenylalanine oxidation.<br><br>Whole-body phenylalanine is calculated from the decarboxylation rate which is assessed by the infusion of stable isotope labelled phenylalanine and tyrosine, detection of its amounts in plasma of the patients by mass spectrometry and kinetic modelling.[Whole-body phenylalanine oxidation will be measured just before (baseline) and at 3 and 24 hours after start of the intervention.];Plasma amino acid profile.<br><br>Plasma amino acid profile is measured by established HPLC technique on plasma samples from the patients. [Plasma amino acid profile is measured just before (baseline) and at 3 and 24 after start of the infusion.]
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