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Comparison of Arterolane-piperaquine Versus Arterolane-piperaquine+Mefloquine Versus Artemether-lumefantrine in Kenyan Children

Phase 3
Completed
Conditions
Plasmodium Falciparum
Interventions
Drug: Artemether-lumefantrine
Drug: Arterolane-piperaquine+mefloquine
Drug: Arterolane-piperaquine
Registration Number
NCT03452475
Lead Sponsor
University of Oxford
Brief Summary

This open-label randomised controlled clinical trial will compare the safety, tolerability, therapeutic efficacy and pharmacokinetics and pharmacodynamics of arterolane-piperaquine, arterolane-piperaquine plus mefloquine versus artemether-lumefantrine.in children with uncomplicated falciparum malaria in Kilifi, Kenya. This study will also provide an up to date insight on the current presence of antimalarial resistance in this site.

In addition, all children will be treated with a single low dose of primaquine, dosing is age based.

The investigators will recruit 219 patients aged 2 years to 12 years with acute uncomplicated falciparum malaria in Kilifi County Hospital.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
219
Inclusion Criteria
  1. Male or female aged 2 years to <13-year-old

  2. Uncomplicated falciparum malaria as defined as:

    • Positive blood smear with asexual forms of P. falciparum (may be mixed with non-falciparum species)
    • Parasitaemia between 5,000-250,000 parasites/µL
    • Fever defined as tympanic temperature >37.5°C or history of fever within last 48 hours
  3. Ability to take oral medication

  4. Willingness and ability to comply with study protocol for study duration

  5. Written informed consent given to participate in the trial

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Exclusion Criteria
  1. Signs of severe/complicated malaria*
  2. Any clinical reason suggesting that the child's treatment should be given immediately and not delayed during the transfer to Kilifi County Hospital in the opinion of the treating physician.
  3. Acute illness other than malaria requiring urgent systemic treatment as assessed by the treating physician
  4. Previous splenectomy
  5. Treatment with artemisinin or ACT within the previous 7 days
  6. Treatment with mefloquine in the 2 months prior to presentation
  7. Known hypersensitivity or contraindication to arterolane-piperaquine, DHA-piperaquine, artemisinin, mefloquine (epilepsy, major psychiatric illness) or primaquine
  8. QTc interval >450 milliseconds at point of presentation
  9. Known personal or family history of cardiac conduction problems
  10. Participation within another clinical trial in the previous 3 months
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Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Artemether-lumefantrineArtemether-lumefantrineArtemether-lumefantrine for 3 days
Arterolane-piperaquine+mefloquineArterolane-piperaquine+mefloquineArterolane-piperaquine + mefloquine for 3 days
Arterolane-piperaquineArterolane-piperaquineArterolane-piperaquine for 3 days
Primary Outcome Measures
NameTimeMethod
42-day PCR corrected adequate clinical and parasitological response (ACPR) by day 42 by study arm42 days
Secondary Outcome Measures
NameTimeMethod
Parasite clearance half-life42 days

Parasite clearance half-life is assessed by entering the parasite counts (assessed by microscopy) in the WWARN PCE calculator

Genome wide association with in vivo/in vitro sensitivity parasite phenotypeBaseline

Genome wide association with in vivo/in vitro sensitivity parasite phenotype

Data on recent travel and current location of livingBaseline
Levels of RNA transcription coding for male or female gametocytesBaseline

Levels of RNA transcription coding for male or female gametocytes at admission

Incidence of adverse events concerning markers of hepatic or renal toxicity42 days

Total bilirubin, Alanine transaminase, Aspartate transaminase, Alkaline phosphatase and creatinine will be measured

Pharmacokinetic profiles and interactions (Cmax) of arterolane and partner drugs42 days

Pharmacokinetic profiles and interactions (Cmax) of arterolane and partner drugs

Day 7 drug levels of partner drugs in association with treatment efficacy and treatment arm7 days

Day 7 drug levels of partner drugs in association with treatment efficacy and treatment arm

Proportion of patients with gametocytaemia before, during and after treatment42 days

Proportion of patients with gametocytaemia before, during and after treatment

Incidence of prolongation of the corrected QT interval42 days

Incidence of the prolongation of the corrected QT interval above 500 ms or \> 60 ms above baseline values

Prolongation of the corrected QT intervalBaseline, hour 4, hour 24, hour 28, hour 48 and hour 52

Prolongation of the corrected QT interval compared at hour 4, hour 24, hour 28, hour 48 and hour 52 compared to baseline

Change in haematocritBaseline, hour 24, hour 48, hour 72, day 7, day 14, day 21, day 28, day 35 and day 42

Change in haematocrit at hour 24, hour 48, hour 72, day 7, day 14, day 21, day 28, day 35 and day 42 according to geographical location and study arm, stratified for G6PD status

Proportion of patients that reports completing a full course of observed TACT or ACT42 days

Proportion of patients that reports completing a full course of observed TACT or ACT without withdrawal of consent or exclusion from study because of drug related serious adverse event

A comparison of transcriptomic patterns between sensitive and resistant parasitesBaseline and 6 hours

Transcriptomic patterns measure at baseline and at specified time points after the start of treatment comparing sensitive and resistant parasites

Parasite count to fall 50%42 days

Time for parasite count to fall 50% of initial parasite density

Fever clearance time42 days

The time taken for the tympanic temperature to fall below 37.5˚C and remain there for at least 24 hours

Incidence of clinical adverse events and serious adverse events42 days
Prevalence/incidence of other genetic markers of antimalarial drug resistance such as multidrug resistance gene 1 copy number and multidrug resistance gene 1 mutationsBaseline

Prevalence/incidence of other genetic markers of antimalarial drug resistance such as multidrug resistance gene 1 copy number and multidrug resistance gene 1 mutations

In vitro sensitivity of P. falciparum to artemisinins and partner drugsBaseline and day recurrent infection
Parasite reduction rates24 and 48 hours

Parasite reduction rates and ratios at 24 and 48 hours assessed by microscopy

Parasite count to fall 90%42 days

Time for parasite count to fall 90% of initial parasite density

Prevalence of Kelch13 mutations of known significanceBaseline

Prevalence of Kelch13 mutations of known significance

Trial Locations

Locations (1)

Kilifi County Hospital

🇰🇪

Kilifi, Kenya

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