Effects of Free Fatty Acids and 3-hydroxybutyrate on Protein, Glucose, Lipid Metabolism and Intracellular Signals; a Study on Muscle Wasting and Metabolism During Acute Inflammation and Potential Anabolic Mechanisms.
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 10
- 试验地点
- 1
- 主要终点
- Protein metabolism
研究概览
简要总结
The investigators hypothesize that the investigators can detect protein-sparing effects of administration of the ketone 3-hydroxybutyrate and free fatty acids during simulation of an acute inflammatory disease. The investigators use the infusion of endotoxin, US standard reference E.coli in healthy subjects as a model for inflammation / infection and to evaluate the effect on protein metabolism using different tracers and the investigators can measure the various intracellular signaling pathways of selected muscle and adipose tissue.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 20 Years 至 40 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •20 < body mass index < 30 kg/m
- •written and orally informed consent before enrollment.
排除标准
- •participation in other studies using radioactive tracers or other exposure to radiation (X-rays, scintigraphy etc).
- •allergy to soy products or eggs
- •diabetes, any type
- •ongoing infection
- •immune deficiency
- •cardiovascular disease
- •dysregulated hypertension
- •primary muscles disease, congenital or acquired
研究组 & 干预措施
Acipimox + 3-hydroxybutyrate
Endotoxin + Acipimox + 3-hydroxybutyrate. Intervention: 3-hydroxybutyrate
干预措施: Endotoxin, US standard reference E.coli (Other)
Placebo (isotonic saline)
Endotoxin + isotonic saline. Reference for model of acute inflammatory illness
干预措施: Endotoxin, US standard reference E.coli (Other)
Acipimox + Placebo (isotonic saline)
Endotoxin + Acipimox + Placebo (isotonic saline). Intervention: blockage of endogenous lipolysis.
干预措施: Endotoxin, US standard reference E.coli (Other)
Acipimox + free fatty acids
Endotoxin + Acipimox + free fatty acids. Intervention: free fatty acids
干预措施: Endotoxin, US standard reference E.coli (Other)
结局指标
主要结局
Protein metabolism
时间窗: 6 hours intervention period
Measurements of protein oxidation during intervention using radioactive tracer techniques.
次要结局
- Activated intracellular signalling pathways(study day)