Polar Body Removal Before ICSI as a Method of Oocyte Activation in Previous Fertilization Failure Cases
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 40
- 试验地点
- 2
- 主要终点
- fertilization rate of the zygotes
研究概览
简要总结
Cases which undergone ICSI before with failure of fertilization will do another ICSI cycle with removal of the polar body just before the ICSI procedure and sperm injection at the site of polar body removal. This study group will be evaluated with another group with the classic ICSI procedure
详细描述
Patients with previous fertilization failure will be divided into 2 groups:
Group 1 (control): Will undergo classic ICSI procedure in half the MII oocyte of the same case.
Group 2 (study group): The other half of the MII oocyte of the same case will undergo:
- Polar body removal.
- ICSI procedure with injection of the spermatozoon in the same zona opening (done by laser for polar body removal).
Results: Will be collected with evaluation of number of 2PN zygotes, Day 1, Day 2, Day 3, Day 5 embryos in all the cases.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 40 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •previous fertilization failure
- •previous fertilization arrest
排除标准
- •NO MII oocyte
- •Refusal of the procedure by the couple
研究组 & 干预措施
polar body removal
polar body removal by zona drilling with laser then suction by injecting pipette then ICSI procedure is done
干预措施: polar body removal using zona drilling by laser then suction by injecting pipette (Procedure)
polar body removal
polar body removal by zona drilling with laser then suction by injecting pipette then ICSI procedure is done
干预措施: Classic ICSI (Procedure)
结局指标
主要结局
fertilization rate of the zygotes
时间窗: 16-20 hours post ICSI
fertilization check is done under inverted microscopy by 2PN check
次要结局
- division rate of the embryos by the number of the blastomeres.(Day 2 & 3 & 5)
研究者
Ahmed Saad
Lecturer of Ob.&Gyn.
Benha University