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study to determine safety, tolerability and pharmacokinetics of Ursodiol injection 625mg/25ml (25mg/ml)

Phase 1
Completed
Registration Number
CTRI/2023/04/051381
Lead Sponsor
Shilpa Medicare Limited
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
Completed
Sex
Not specified
Target Recruitment
30
Inclusion Criteria

1. Healthy human subjects between 18-45 years of age (including both) and weight = 50 Kg (BMI 18.00- 25.00 kg/m²).

2. Acceptable findings during registration and screening including, detailed medical history, full physical examination, medical examination, laboratory evaluations, 12- lead ECG and Chest X-Ray (postero-anterior view).

3. Values within Normal Ranges of Laboratory Parameters upon evaluation by the Investigator or Physician.

4. Subjects able to communicate effectively.

5. Subjects willing to give written informed consent and adhere to all the requirements of this protocol.

6. Subject willing to abstain from all kinds of alcoholic beverages, smoking, and caffeine/xanthine containing foods or beverages from 96.00 hours before admission till the last blood sample in study period.

7. Male Subjects:

Subjects willing to follow approved birth

control methods (a double barrier method) for

the duration of the study as judged by the

investigator(s), such as (a double barrier

method) Condom with spermicide, Condom with

diaphragm, or abstinence, Subjects should also not donate

sperm during this time.

8. Female subjects:

Postmenopausal for at least 1 year or

Surgically sterile (bilateral tubal ligation,

bilateral oophorectomy or

hysterectomy has been performed on the subject)

Exclusion Criteria

1. Volunteers having history of contraindication or hypersensitivity (e.g.,

anaphylaxis) to ursodeoxycholic acid or other related drugs, or related group of

drugs.

2. A history or presence of significant asthma, urticaria, or other allergic-type

reactions after taking aspirin or other NSAIDs. Severe, rarely fatal, anaphylacticlike

reactions to NSAIDs, seizures, diabetes, migraine, hypertension,

cardiovascular, pulmonary, neurological or psychiatric disease/disorder,

dermatological, endocrine, eye disorders, immunological, renal, hematopoietic,

gastrointestinal, ongoing infectious diseases, or any other significant

abnormality as evidenced by medical history and physical examination or

according to the opinion of the physician.

3. History or presence of gastrointestinal (GI) inflammation, bleeding, ulceration,

and perforation of the stomach, small intestine, or large intestine.

4. History or presence of hepatic, thyroid, or renal dysfunction.

5. History or presence of bronchial asthma.

6. History or evidence of exfoliative dermatitis, Stevens - Johnson syndrome (SJS),

and toxic epidermal necrolysis (TEN).

7. Any known enzyme inducing or inhibiting drug taken within 30 days before the

study.

8. A depot injection or implant of any drug within 3 months prior to the dose of

study medication.

9. Use of CYP enzyme inhibitors or inducers within 30 days prior to the dose of

study medication

10. Participation in a drug research study within 90 days prior to dosing of this study.

11. Blood loss or whole blood donation within 90 days prior to drug administration.

12. Current use or anticipated need for drugs with known or suspected interactions

with ursodeoxycholic acid.

13. Subjects with hypertension or cardiovascular risk factors.

14. Consumption of high caffeine (more than 5 cups of coffee or tea/day).

15. Smokers who smoke 10 or more cigarettes per day or 20 or more biddies per day

or those who cannot refrain from smoking during the study period.

16. History of addiction to any recreational drug or drug dependence.

17. An unusual or abnormal diet, for whatever reason within 96.00 hours prior to

admission of study period, e.g. fasting due to religious reasons.

18. History of dehydration from diarrhea, vomiting or any other reason within a

period of 96.00 hours prior to study admission of study period.

19. Positive results for drugs of abuse (benzodiazepines, cocaine, opioids,

amphetamines, cannabinoids and barbiturates) in urine during the study

admission of study period.

20. Positive results for alcohol consumption tests during the study admission.

21. History of pre-existing bleeding disorder.

22. Difficulty with donating blood.

23. Systolic blood pressure less than 100 mm Hg or more than 140 mm Hg.

24. Diastolic blood pressure less than 60 mm Hg or more than 90 mm Hg.

25. Pulse rate less than 60 beats/minute or more than 100 beats/minute.

26. Use of any prescribed medication or OTC medicinal products and herbal

medicines during last two weeks preceding the first dosing.

27. Female volunteer who is pregnant, currently breast-feeding.

28. Female subjects demonstrating a positive pregnancy test.

29. Female vo

Study & Design

Study Type
Interventional
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
1. To evaluate safety and tolerability of single ascending doses of Ursodiol injection 625mg/25ml (25mg/ml) (600 mg, 900 mg, <br/ ><br>1800 mg & 3500 mg) as intravenous infusion over one hour in healthy adult human subjects. <br/ ><br>2. To determine the maximum tolerated dose (MTD)Timepoint: Tolerability of Test product dose will be assessed after each cohort (15 days)
Secondary Outcome Measures
NameTimeMethod
To determine single dose pharmacokinetics of Ursodiol injection 625mg/25ml (25mg/ml) as IV infusion. To assess the dose proportionality of ascending doses of Ursodiol <br/ ><br>injection 625 mg/25 ml (25mg/ml) as IV infusion up to 3500 mg. To compare the pharmacokinetics of ursodeoxycholic acid administered as IV infusion with orally administered Ursodiol CapsulesTimepoint: Blood samples (6.0 mL each) will be collected at -48.00, -42.00, - 36.00, -30.00, -24.00, -18.00, -12.00, -06.00, pre-dose (00.00) hours prior to dosing and at 0.25 (15 minutes after start of infusion), 00.50 <br/ ><br>(30 minute after start of infusion), 00.75, 00.91 (5 minutes prior to end of infusion), 01.00, 01.17, 01.50, 0l .75, 02.00, 02.50, 03.00, 04.00, 05.00, 06.00, 08.00, 10.00, 12.00, 16.00, 20.00, 24.00, 36.00, 48.00, 96.00, 144.00,216.00,288.00 and 360.00 hours post dose.
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