Impact of Beetroot Supplementation on Sports Performance, Prooxidant-antioxidant Balance and Sleep in Athletes
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 20
- 试验地点
- 2
- 主要终点
- Objective Sleep - Light sleep
研究概览
简要总结
Several previous studies observed a positive effect of beetroot supplementation on sports performance in athletes, mainly in endurance performance. However, less attention has been paid to its effects on short-duration performance, especially in youth athletes. Moreover, only a few previous investigations performed biochemical analyses, including pro-oxidant balance assessments. It is especially important because chronic nitrate intake might directly cause the production of reactive nitrogen species and reactive oxygen species in other subcellular compartments, leading to more oxidative stress. Additionally, no previous study verified the impact of beetroot intake on sleep.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Treatment
- 盲法
- Double (Participant, Investigator)
入排标准
- 年龄范围
- 14 Years 至 23 Years（Child, Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •good general health assessed by physician
- •highly trained
- •written consent to participate
排除标准
- •medication or dietary supplements used within the previous month, which could potentially impact the study outcomes
- •history of sleep nor neurological disorders
- •chronic use (6 months) of any antibacterial mouthwash products.
结局指标
主要结局
Objective Sleep - Light sleep
时间窗: nights 1 - 7 during treatment
Total amount of time in light sleep assessed by sleep radar
Objective Sleep - Deep sleep
时间窗: nights 1 - 7 during treatment
Total amount of time in deep sleep assessed by sleep radar
Objective Sleep - REM sleep
时间窗: nights 1 - 7 during treatment
Total amount of time in REM sleep assessed by sleep radar
Performance test
时间窗: 7 days after treatment
yo-yo intermittent recovery test level 1
Changes in nitrites
时间窗: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
Blood plasma will be obtained to measure nitrite concentration
Changes in nitrates
时间窗: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
Blood plasma will be obtained to measure nitrate concentration
Changes in antioxidant status
时间窗: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
Blood plasma will be obtained to measure total antioxidant capacity
Changes in prooxidant status
时间窗: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
Blood plasma will be obtained to measure total oxidative status
Changes in oxidative stress index
时间窗: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
The oxidative stress index will be calculated as the ratio of plasma prooxidant to antioxidant status
Changes in protein oxidation
时间窗: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
Blood plasma will be obtained to measure advanced protein oxidation products concentration
Changes in exercise-induced metabolic acidosis
时间窗: 1) Before the performance test 2) 3 minutes after the performance test 3) 10 minutes after the performance test 4) 20 minutes after the performance test
Arterialized blood will be obtained to measure lactate concentration
Objective Sleep - Total sleep time
时间窗: nights 1 - 7 during treatment
Total sleep time obtained from sleep onset to time at wake-up assessed by sleep radar
Changes in nitrooxidative stress markers
时间窗: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
Blood plasma will be obtained to measure 3-nitrotyrosine concentration
Changes in nucleic acid oxidation
时间窗: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
Blood plasma will be obtained to measure ox-8-hydroxy-2'-deoxyguanosine (8-OHdG) concentration
Changes in lipids oxidation
时间窗: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
Blood plasma will be obtained to measure thiobarbituric acid reactive substances concentration
Objective Sleep - Time in bed
时间窗: nights 1 - 7 during treatment
Total time spent in bed during the night assessed by sleep radar
Objective Sleep - Sleep-onset latency
时间窗: nights 1 - 7 during treatment
The time it takes from when the athlete intends to go to sleep and actually starts to sleep assessed by sleep radar
Objective Sleep - Sleep efficiency
时间窗: nights 1 - 7 during treatment
The percentage of total sleep time to lights off and leaving bed assessed by sleep radar
次要结局
- Countermovement jump height(7 days after treatment)
- Changes in muscle enzymes activity(1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test)
- Changes in blood count(1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test)
- Changes in catabolic hormones(1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test)
- Changes in the purine metabolism marker(1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test)
- Changes in muscle damage markers(1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test)
- Maximal isometric handgrip strength(7 days after treatment)
- Changes in inflammation markers(1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test)
- Changes in anabolic hormones(1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test)
- Changes in plasma volume(1) Before the performance test 2) 3 minutes after the performance test 3) 10 minutes after the performance test 4) 20 minutes after the performance test)
- Changes in kidney function markers(1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test)
- Changes in hemoglobin concentration(1) Before the performance test 2) 3 minutes after the performance test 3) 10 minutes after the performance test 4) 20 minutes after the performance test)
- Changes in hematocrit(1) Before the performance test 2) 3 minutes after the performance test 3) 10 minutes after the performance test 4) 20 minutes after the performance test)
- Ball speed velocity(7 days after treatment)
- Sprint test (0-20m)(7 days after treatment)
- Agility T-test(7 days after treatment)
- Flanker task(7 days after treatment)