MedPath

Early Life Interventions for Childhood Growth and Development In Tanzania

Phase 2
Completed
Conditions
Malnutrition
Stunting
Cognitive Development
Enteric Pathogens
Interventions
Registration Number
NCT03268902
Lead Sponsor
Haydom Lutheran Hospital
Brief Summary

This study aims to assess growth and cognitive effects of treatment with azithromycin and nitazoxanide and/or nicotinamide (vitamin B3) supplementation nicotinamide.

Detailed Description

Children living in rural sub-Saharan Africa experience massive challenges to child thriving, with poor linear growth and delays in child development. In a cohort of 211 children living in the rural Haydom area of Tanzania (participating in the Interactions of Malnutrition \& Enteric Infections: Consequences for Child Health and Development "MAL-ED" Study), 70.6% had stunted growth at 18 months. This rate of moderate and severe stunting (length-for-age z-score \[HAZ\] \<-2 standard deviations) was the highest of the 8 study sites in MAL-ED.

This enormous deficit is likely associated with high rates of enteric infections with Campylobacter, E. coli pathotypes, Cryptosporidium, and Giardia, organisms susceptible to azithromycin and/or nitazoxanide. Infections such as these occur frequently in developing areas and are often associated with environmental enteropathy, including ongoing enteric inflammation and loss of enterocyte integrity, leading to possible bacterial translocation and poorer absorption of ingested nutrients. The consequences of these infections, enteric dysfunction and poor nutrient absorption frequently include growth stunting, learning delays, and an overall loss of human capital.

Emerging evidence suggests a potential role for the tryptophan-niacin pathway (including the end-product nicotinamide, an isoform of vitamin B3) in decreasing mucosal inflammation and affecting enteral microbiota. At the Tanzania site of MAL-ED, serum levels of tryptophan were related to subsequent linear growth, further suggesting importance of the tryptophan-niacin pathway. What is not clear is whether early childhood growth and development could be improved by targeting enteric infection and the tryptophan-niacin pathway by 1) delivering antibiotics against specific bacteria and/or 2) providing vitamin B3 as nicotinamide/niacinamide.

The main analysis will be intention-to-treat but a secondary analysis will be per protocol.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
1188
Inclusion Criteria
  1. Maternal age ≥18
  2. Infant ≤ 14 days
Exclusion Criteria
  1. Maternal inability to adhere to protocol
  2. Multiple gestation
  3. Severe illness (significant birth defect, hospitalization, severe neonatal illness)
  4. Birth weight <1500 g
  5. Lack of breastfeeding at enrollment (and lack of intention to continue breastfeeding at time of enrollment).

Study & Design

Study Type
INTERVENTIONAL
Study Design
FACTORIAL
Arm && Interventions
GroupInterventionDescription
Nicotinamide and AntimicrobialsAzithromycin Oral Liquid ProductNicotinamide Azithromycin Oral Liquid Product Nitazoxanide Oral Suspension
Nicotinamide and AntimicrobialsNitazoxanide Oral SuspensionNicotinamide Azithromycin Oral Liquid Product Nitazoxanide Oral Suspension
Nicotinamide and AntimicrobialsNicotinamideNicotinamide Azithromycin Oral Liquid Product Nitazoxanide Oral Suspension
Antimicrobials onlyAzithromycin Oral Liquid ProductPlacebo Azithromycin Oral Liquid Product Nitazoxanide Oral Suspension
Antimicrobials onlyNitazoxanide Oral SuspensionPlacebo Azithromycin Oral Liquid Product Nitazoxanide Oral Suspension
Antimicrobials onlyPlacebosPlacebo Azithromycin Oral Liquid Product Nitazoxanide Oral Suspension
Nicotinamide onlyNicotinamideNicotinamide Placebo Placebo
Nicotinamide onlyPlacebosNicotinamide Placebo Placebo
No active treatmentPlacebosPlacebo Placebo Placebo
Primary Outcome Measures
NameTimeMethod
Height-for-age z-score (HAZ) at 18 months18 months
Secondary Outcome Measures
NameTimeMethod
Stunting18 months

HAZ \<-2

All cause mortality0-18 months
Weight-for-age z-score (WAZ) at 18 months18 months
Head circumference-for-age z-score (HCAZ) at 18 months18 months
Hospitalization0-18 months
Childhood illness0-18 months

Incidence of diarrhea, lower respiratory infection and febrile illness

Anemia12 and 18 months

Moderate to severe anemia by WHO definition for age and altitude

Enteropathogen burden6, 6.5, 12, 12.5, 18 months
Microbiota composition6, 6.5, 12, 18 months

Composition of intestinal microbiome

Stool myeloperoxidase concentration6, 12, 18 months

Stool myeloperoxidase ELISA

C-reactive protein concentration in serum12 and 18 months

High-sensitivity CRP concentration

Insulin-like growth factor 1 concentration in serum12 and 18 months
Collagen X concentration in serum12 and 18 months
Tryptophan-kynurenine ratio12 and 18 months

Ratio of tryptophan concentration to kynurenine concentration in metabolomic testing

Niacin and nicotinamide metabolite concentration6, 12, 18 months

Concentration of downstream metabolites of niacin and nicotinamide as tested by metabolomic analysis

Small intestinal bacterial overgrowth6, 12 and 18 months

Prevalence of SIBO as tested via exhaled hydrogen

Malawi Developmental Assessment Tool score18 months

The MDAT is a measure of child cognitive development

Trial Locations

Locations (1)

Haydom Lutheran Hospital

🇹🇿

Haydom, Manyara, Tanzania

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