Efficacy of Concentrated Growth Factor (CGF) in enhancing soft tissue healing in active smokers.

The purpose of this research is to evaluate the efficacy of concentrated growth factor  in promoting soft tissue healing and preventing alveolar osteitis in active smokers.

Patients reporting to the outpatient department of PMS College of Dental Science and Research indicated for surgical extraction of mandibular third molar who fulfill the inclusion criteria will be included in the study.

Informed consent will be obtained from the patient.

After surgical removal of mandibular 3rd molar, CGF in gel form will be inserted into the socket in the study group. Absorbable gelatin sponge will be placed in the socket of the control group.

CGF will be prepared by collecting two 10 mL blood samples from the patient. The tubes will be centrifuged in centrifuge device immediately to prevent coagulation of blood. The guidelines of centrifugation to be followed are acceleration for 30 sec so as to reach 2700 rpm, rotated for 2 mins, then reduced to 2400 rpm, for 4 mins and then accelerated to 2700 rpm for 4 mins, then accelerated to 3000 rpm for 3 mins, and decelerated for 36 sec to stop.

3 layers that will be obtained in the tube are

1)Upper platelet poor plasma

2)Middle buffy coat containing CGF fibrin gel

3)Lower red blood cells

The middle buffy coat containing the CGF layer will be taken out of the tube and split from the red blood cells.

CGF fibrin gel will be then placed into the patient’s extraction socket and wound closure completed with silk suture.

Every patient will be recalled on day 4 day of surgery to evaluate healing progress. The onset of alveolar osteitis will be recorded based on the clinical appearance of the socket that will be quantified on a scoring system elaborated below.10 Sutures will be removed at day10 after surgery. Soft tissue healing will be evaluated.