Effects of exercise mode on human skeletal muscle and circulating biological networks

Not Applicable

Completed

• Conditions: Metabolic disorders; Obesity; Metabolic and Endocrine - Metabolic disorders; Diet and Nutrition - Obesity

• Registration Number: ACTRN12619000819123
• Lead Sponsor: Professor John Hawley

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Study Start: 2019-06-06
• Study Completion: 2019-11-29
• Last Update Posted: 13 January 2020

Recruitment & Eligibility

• Status: Completed
• Sex: Male
• Target Recruitment: 10

Inclusion Criteria

Males; 18­ - 30 years; BMI 18.5 - ­24.9 kg/m2; sedentary and have not performed structured physical activity for 6 months prior to recruitment; no cardiopulmonary abnormalities; no injuries; apparently healthy; ability to pass the ESSA pre-exercise screening tool and/or obtain general practitioner clearance to exercise; able to ride a stationary cycle ergometer at high intensity

Exclusion Criteria

Known cardiovascular disease or diabetes mellitus; known gastrointestinal disorder; previous bariatric surgery; major or chronic illness that impairs mobility or eating/digestion; known transmissible disease [e.g. hepatitis, HIV]; known bleeding disorder (i.e. hemophilia A [factor VIII deficiency], hemophilia B [factor IX deficiency], von Willebrand disease, or other rare factor deficiencies including I, II, V, VII, X, XI, XII and XIII); taking prescription medications (i.e. beta-blockers, anti-arrhythmic drugs, statins, insulin sensitising drugs, or drugs that increase the risk of bleeding [i.e. anticoagulants, antiplatelets, novel oral anticoagulants [NOAs], nonsteroidal anti-inflammatory drugs [NSAIDs], selective norepinephrine reuptake inhibitors [SNRI], or selective serotonin reuptake inhibitors [SSRIs]]); habitual physical activity of greater than or equal to 150 minutes per week of moderate-intensity exercise; previous bariatric surgery; smokers; current restriction of dietary intake (i.e. actively trying to diet and lose weight); not being weight stable (+/- 5 kg) for the last 3 months.

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Determine the effects of a single bout of HIIT versus continuous endurance exercise on skeletal muscle protein post-translational modifications (i.e. phosphorylation status).[Skeletal muscle biopsy samples will be obtained before, during, immediately following, 1 hour post and 3 hour post HIIT and endurance exercise protocols to measure skeletal muscle protein post-translational modifications (i.e. phosphorylation status). Each participant will complete the two exercise protocols on two trial days separated by a minimum of 10 days.];Determine the effects of a single bout of HIIT versus continuous endurance exercise on skeletal muscle protein content.[Skeletal muscle biopsy samples will be obtained before, during, immediately following, 1 hour post and 3 hour post HIIT and endurance exercise protocols to measure skeletal muscle protein content. Each participant will complete the two exercise protocols on two trial days separated by a minimum of 10 days.]

Secondary Outcome Measures

Name	Time	Method
Determine the effects of a single bout of HIIT versus continuous endurance exercise on regulation of skeletal muscle gene expression (i.e. transcription).[Skeletal muscle biopsy samples will be obtained before, during, immediately following, 1 hour post and 3 hour post HIIT and endurance exercise protocols to measure skeletal muscle gene expression and regulation of transcription. Each participant will complete the two exercise protocols on two trial days separated by a minimum of 10 days.];Determine the effects of a single bout of HIIT versus continuous endurance exercise on plasma hormone and metabolite concentrations. This is a composite and exploratory secondary outcome. [Blood samples will be obtained before, during, immediately following, 1 hour post and 3 hour post HIIT and endurance exercise protocols to measure plasma hormone and metabolites. Each participant will complete the two exercise protocols on two trial days separated by a minimum of 10 days.]