Hemodialysis and Erythrocyte Fatty-Acid Status: a Lipidomics Study
- Conditions
- LipidomicsFatty Acid MetabolismErythrocytesHemodialysis
- Interventions
- Other: Blood sampling
- Registration Number
- NCT03857984
- Lead Sponsor
- Benjamin Gollasch
- Brief Summary
Hypotension with potentially serious consequences for organ perfusion is a common complication in extracorporeal procedures such as hemodialysis / hemofiltration. The exact reasons for this are still insufficiently clarified. Probably periinterventional vasorelaxant released substances play a crucial role in these procedures. These substances could be due to contact of blood cells with the Membrane in the dialyzer / hemofilter arise. In this project the hypothesis will be checked, if EETs / DHETs are released by Erythrocytes during hemodialysis and thus act as potential candidate products for the result of hypotonic phases in the Dialysis.
We will determine differences in RBC fatty acids profiling in hemodialysis patients before and after dialysis intervention. Furthermore a matched control group of healthy individuals will be profiled. RBC fatty acids profiling will be achieved by using targeted HPLC-MS mass spectrometry.
It is believed that during hemodialysis / HLM / CVVH there is an increase in EETs / DHETs in the serum and in the erythrocytes. It is believed that shear forces play an important role in the release of erythrocyte EETs / DHETs.
- Detailed Description
Differences in Erythrocyte Fatty-Acid Status Profiling in:
* before and after hemodialysis intervention
* in hemodialysis patients and healthy individuals Venous blood will be collected from each subject in the sitting position
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 30
Not provided
Not provided
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- SINGLE_GROUP
- Arm && Interventions
Group Intervention Description Erythrocyte Fatty-Acid Status Blood sampling Erythrocyte Fatty-Acid Status Profiling of HD patients are studied before and after a single HD using LC-MS / MS
- Primary Outcome Measures
Name Time Method Coefficient of variation of relative quantities of erythrocyte fatty-acid parameters related to its dynamic during Hemodialysis, using LC-MS / MS. 4-5 Months EETs / DHETs will be analyzed in the erythrocytes and serum of the recovered blood samples.
The determination will made in cooperation with a Berliner Biotech company. This involves liquid chromatography with MS coupling (LC-MS / MS) used. The data analysis is carried out by means of suitable statistical methods to perform erythrocyte fatty-acid status profiling.
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
Charité University Experimental & Clinical Research Center
🇩🇪Berlin, Germany