The Exploratory Study on the Baseline Level of NAD+/NADH in Peripheral Blood Samples of Different Gender and Age Groups of Healthy Adults.
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 入组人数
- 60
- 试验地点
- 1
- 主要终点
- The baseline in NAD+ and NADH concentrations in whole blood of healthy adults.
研究概览
简要总结
This study investigates the concentration levels of NAD+/NADH in peripheral blood samples and immune cells of healthy adults of different age groups under daily physiological and lifestyle conditions, in order to determine the baseline levels of NAD+/NADH in adult populations of different age groups and genders. Simultaneously examining the biological status and function of human immune cells, blood biochemistry, biological age levels, to explore the biological function and mechanism of NAD+/NADH in the aging process of the human body.
详细描述
In this study, 60 subjects will be enrolled in three groups according to the age range of 20-40, 41-60 and 61-80. Each group has 20 subjects, and the sex ratio of each group is 1:1. After each participant is enrolled, peripheral blood samples are taken before breakfast and before lunch to detect NAD+/NADH levels in peripheral blood and determine the physiological baseline levels of NAD+/NADH in different age groups. At the same time, the subjects tested Hematology, blood biochemical indicators, as well as the expression of subsets of peripheral blood immune cells and SIRT1 level at the same time point, to study the change trend of NAD+/NADH level in different age groups, and the correlation between this trend and Hematology age, immune indicators.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Other
入排标准
- 年龄范围
- 18 Years 至 85 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Male/females of 18 to 85 years of age.
- •Able to provide written Informed Consent.
- •Body Mass Index ranging from 18 to 25 kg/m2(bounds included), with a stable weight over the last 2 months (±2.5 kg).
- •Able to follow verbal and written study directions.
- •Must not be taking or be willing to take any supplements containing any form of niacin for seven days prior to baseline and for the duration of the study.
- •6.Able to maintain consistent diet and lifestyle habits according to the study
排除标准
- •Male/females of 18 to 85 years of age.
- •Able to provide written Informed Consent.
- •Body Mass Index ranging from 18 to 25 kg/m2(bounds included), with a stable weight over the last 2 months (±2.5 kg).
- •Able to follow verbal and written study directions.
- •Must not be taking or be willing to take any supplements containing any form of niacin for seven days prior to baseline and for the duration of the study.
- •Able to maintain consistent diet and lifestyle habits according to the study.
结局指标
主要结局
The baseline in NAD+ and NADH concentrations in whole blood of healthy adults.
时间窗: Fasting at 0 hour, 4 hour on the day of the trial
Monitor
Counting of blood cell indicators such as red blood cells, white blood cells, and platelets in healthy adults
时间窗: Fasting at 0 hour on the day of the trial
Monitor
Blood biochemical indicators of the healthy adults monitoring including liver function; lood lipids; blood sugar; renal function; uric acid; lactate dehydrogenase; creatine kinase, etc
时间窗: Fasting at 0 hour on the day of the trial
Monitor
Peripheral blood immune cell phenotype of the healthy adults monitoring including NK cells, B lymphocytes, eosinophils, T lymphocytes, platelet neutrophils, basophils, and monocytes, etc
时间窗: Fasting at 0 hour on the day of the trial
Monitor
次要结局
未报告次要终点