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Therapeutic Cancer Vaccine (AST-301, pNGVL3-hICD) in Gastric Cancer

Phase 2
Recruiting
Conditions
Gastric Cancer
Interventions
Registration Number
NCT05771584
Lead Sponsor
Aston Sci. Inc.
Brief Summary

The purpose of this early proof-of-concept study to evaluate the safety and immunologic efficacy of AST-301 in gastric cancer patients with HER2 expression (including both HER2 low expression and overexpression) who have completed the standard adjuvant treatment (including those who discontinued the standard adjuvant treatment due to intolerance).

Participants will be randomized 1:1 to either Arm 1 (Q3W, 3 cycles), or Arm 2 (Q3W, 6 cycles) of the study.

Safety Monitoring Committee (SMC) will oversee safety of study at 25% (6 participants), 50% (12 participants), and 75% (18 participants) of participants receive at least 1 dose of AST-301 and survival follow up will be performed to determine disease-free survival (DFS).

Detailed Description

Participants will provide informed consent and will undergo Pre-Screening/Screening procedures before taking part in the study. Participants will be in Arm 1 and Arm 2 will be conducted in parallel. AST-301 will be administrated every 3 weeks for a total of 3 immunizations in Arm 1 and a total of 6 immunizations in Arm 2.

* Arm 1: 3 immunizations of AST-301 admixed with immunoadjuvant recombinant human granulocyte-macrophage colony stimulating factor (rhuGM-CSF) administered at 3-week intervals. (Total 300 μg of AST-301)

* Arm 2: 6 immunizations of AST-301 admixed with immunoadjuvant recombinant human granulocyte-macrophage colony stimulating factor (rhuGM-CSF) administered at 3-week intervals. (Total 600 μg of AST-301) Randomization will be stratified according to HER2 expression (HER2 low expression or HER2 overexpression).

For both Arm 1 and Arm 2 of the study there will be a Pre-screen period, followed by study periods: a Screening Period (Day -28 to Day -1), a Treatment Period (3 cycles/Arm 1 and 6 cycles/Arm 2), an end of treatment (EOT) visit and follow-up visits.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
24
Inclusion Criteria
  • Underwent a curative surgery with standard lymph node dissection (confirmed with no residual tumor, R0 resection) and have completed standard adjuvant treatment
  • Has stages II or III according to the 8th edition of the American Joint Committee on Cancer (AJCC)
  • HER2 low expression and HER2 overexpression diagnosed according to the 2016 College of American Pathologists (CAP)/American Society for Clinical Pathology (ASCP)/American Society of Clinical Oncology (ASCO) guidelines
  • Eastern Cooperative Oncology Group (ECOG) performance status of 0 to 1
  • Demonstrates adequate organ function.

Key

Exclusion Criteria
  • Has a history of hypersensitivity or other contraindications to rhuGM-CSF
  • Has a history of other malignancies ≤5 years prior to first administration of Investigational Product (IP) except for adequately treated non-melanoma skin cancer or epithelial carcinoma without evidence of disease.
  • Has received systemic immunosuppressants or were treated with systemic immunosuppressants ≤4 weeks prior to the first administration of Investigational Product (IP).
  • Has a history of autoimmune disease or inflammatory disease
  • Has active infection including tuberculosis, hepatitis B, hepatitis C or human immunodeficiency virus (HIV) infection
  • Is pregnant or breastfeeding or expecting to conceive children

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Total 300 μg of AST-301AST-301AST-301/rhuGM-CSF (3-week interval, 3 cycles in total)
Total 300 μg of AST-301rhuGM-CSFAST-301/rhuGM-CSF (3-week interval, 3 cycles in total)
Total 600 μg of AST-301AST-301AST-301/rhuGM-CSF (3-week interval, 6 cycles in total)
Total 600 μg of AST-301rhuGM-CSFAST-301/rhuGM-CSF (3-week interval, 6 cycles in total)
Primary Outcome Measures
NameTimeMethod
Number of participants with treatment-related adverse events as assessed by the National Cancer Institute Common Terminology Criteria for Adverse Events [NCI CTCAE] version 5.0.up to 20 weeks

To assess the safety of AST-301 administered in gastric cancer patients.

Immunologic efficacy of AST-301 immunization52 weeks

AST-301 specific interferon (IFN)-gamma enzyme-linked immune absorbent spot (ELISpot) assay

Secondary Outcome Measures
NameTimeMethod
1year Disease-Free Survival rate (DFS rate)12 months

disease free survival rate at 1 year

Change in central memory T-cell populations between Arm 1 and Arm 252 weeks

Central memory T-cell (cluster of differentiation 4 (CD4) + and cluster of differentiation 8 (CD8) +) by a flow cytometry analysis

Compare immunogenicity of AST-301 between Arm 1 and Arm 252 weeks

AST-301-specific IFN γ response by ELISpot assay

Disease-Free Survival rate (DFS rate) at end of study (EOS)Overall study period approximately 31 months

disease free survival at end of study

Trial Locations

Locations (6)

Kaohsiung Medical University Chung-Ho Memorial Hospital

🇨🇳

Kaohsiung, Taiwan

Chi Mei Medical Center

🇨🇳

Tainan, Taiwan

Taipei Veterans General Hospital

🇨🇳

Taipei, Taiwan

Chang Gung Memorial Hospital Linkou

🇨🇳

Taoyuan, Taiwan

China Medical University Hospital

🇨🇳

Taichung, Taiwan

Taichung Veterans General Hospital

🇨🇳

Taichung, Taiwan

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