Sperm Selection and Embryo Development: Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods Sperm Selection and Embryo Development: Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods

Not Applicable

Completed

• Conditions: ICSI; Reduced Sperm Motility; IVF; Astheno Teratozoospermia
• Interventions: Device: Density gradient; Device: Microfluidic sperm sorting chip

• Registration Number: NCT04962074
• Lead Sponsor: Maltepe University

Brief Summary

Purpose: To compare the effects of the density gradient centrifugation and microfluidic chip sperm preparation methods on embryo development in a study population with astheno-teratozoospermia.

Materials and Methods: In this prospective study, the semen samples of the patients with astheno-teratozoospermia were divided into two groups for preparation with either the microfluidic or density gradient methods. Selected spermatozoa were used to fertilize the mature sibling oocytes. Semen parameters and embryo development on days 3 and 5 were assessed.

Detailed Description

Not available

Study Timeline

• Study Start: 2020-01-05
• Primary Completion: 2020-09-20
• Study Completion: 2020-09-20
• Study First Submitted: 2021-06-25
• Status Verified: 2021-07
• Study First Submitted QC: 2021-07-05
• Last Update Submitted: 2021-07-05
• Study First Posted: 2021-07-14
• Last Update Posted: 2021-07-14

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 22

Inclusion Criteria

• Infertile men with astheno-teratozoospermia
• Women were aged from 18 to 39
• Women had at least two mature oocytes.

Exclusion Criteria

• MI oocytes

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Density gradient method	Density gradient	Sperm prepared by density gradient method
Microfluidic chip	Microfluidic sperm sorting chip	Sperm prepared by microfluidic chip method

Primary Outcome Measures

Name	Time	Method
Semen parameters after sperm preparation with either method: semen volume	up to 7 months	semen volume (ml)
Semen parameters after sperm preparation with either method: total motility	up to 7 months	total motility (%)
Semen parameters after sperm preparation with either method: progressive motility	up to 7 months	progressive motility (%)
Embryo development after sperm preparation with either method	up to 7 months	Embryo development and blastocyst formation were evaluated on days 1, 3 and 5 based on the criteria reported by Veeck and Zaninovic, 2003
Semen parameters after sperm preparation with either method: sperm concentration	up to 7 months	sperm concentration (106/ml)

Trial Locations

Locations (1)

MaltepeU; 🇹🇷 Istanbul, Turkey