Nitrate Supplementation; Source

Phase 4

Completed

• Conditions: Blood Pressure; Nutritional Physiological Phenomena
• Interventions: Dietary Supplement: Sodium nitrate; Dietary Supplement: Beetroot juice; Dietary Supplement: Spinach; Dietary Supplement: Rocket salad

• Registration Number: NCT02271633
• Lead Sponsor: Maastricht University Medical Center

Brief Summary

The main aim of the current study will be to compare the effects of ingesting different nitrate-rich sources on plasma nitrite concentrations.

Detailed Description

Oral ingestion of nitrate (NO3-) has been shown to increase plasma nitrate and nitrite levels. Significant lowering of pulmonary oxygen uptake during exercise, as well as significantly lower blood pressure values at rest have been observed after supplementation with nitrate. However, no study has yet described a direct comparison between different nitrate-rich beverages with respect to the bioavailability of nitrate/nitrite and the possible hemodynamic effects. Based on the gaps in current literature, our main goal will therefore be to gain further insight into the effects of nitrate supplementation with different sources on plasma nitrite levels.

Study Timeline

• Study Start: 2014-09
• Study First Submitted: 2014-10-13
• Study First Submitted QC: 2014-10-20
• Study First Posted: 2014-10-22
• Primary Completion: 2014-12
• Status Verified: 2015-05
• Study Completion: 2016-03
• Last Update Submitted: 2016-03-15
• Last Update Posted: 2016-03-16

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 30

Inclusion Criteria

• 18.5 < BMI < 30 kg/m2
• Engagement in regular physical activity ( > 1 h a week)

Exclusion Criteria

• Use of medication
• Smoking
• Currently supplementing diet with nitrate
• Lactose intolerance

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Sodium nitrate	Rocket salad	Dietary supplement: 800 mg of nitrate in sodium nitrate added with water to get a 140 mL solution (BASF, Ludwigshafen, Germany)
Spinach	Beetroot juice	Dietary supplement: 800 mg of nitrate in a spinach based beverage
Rocket salad	Beetroot juice	Dietary supplement: 800 mg of nitrate in a rocket salad based beverage
Beetroot juice	Rocket salad	Dietary supplement: 800 mg of nitrate in concentrated beetroot juice (Beet-IT)
Spinach	Sodium nitrate	Dietary supplement: 800 mg of nitrate in a spinach based beverage
Rocket salad	Sodium nitrate	Dietary supplement: 800 mg of nitrate in a rocket salad based beverage
Beetroot juice	Sodium nitrate	Dietary supplement: 800 mg of nitrate in concentrated beetroot juice (Beet-IT)
Sodium nitrate	Beetroot juice	Dietary supplement: 800 mg of nitrate in sodium nitrate added with water to get a 140 mL solution (BASF, Ludwigshafen, Germany)
Sodium nitrate	Spinach	Dietary supplement: 800 mg of nitrate in sodium nitrate added with water to get a 140 mL solution (BASF, Ludwigshafen, Germany)
Beetroot juice	Spinach	Dietary supplement: 800 mg of nitrate in concentrated beetroot juice (Beet-IT)
Spinach	Rocket salad	Dietary supplement: 800 mg of nitrate in a spinach based beverage
Rocket salad	Spinach	Dietary supplement: 800 mg of nitrate in a rocket salad based beverage

Primary Outcome Measures

Name	Time	Method
Plasma nitrite levels	Baseline until 5 hours after ingestion	Plasma nitrite levels following ingestion of dietary nitrate

Secondary Outcome Measures

Name	Time	Method
Resting blood pressure	Baseline, 2.5 hours and 5 hours post-ingestion	Resting blood pressure measures following ingestion of dietary nitrate
Plasma levels of nitrate	Baseline until 5 hours post-ingestion	Plasma nitrate levels following ingestion of dietary nitrate
Tongue scraping to assess bacterial mouth flora.	5 h post-ingestion	Tongue scraping to assess bacterial mouth flora.

Trial Locations

Locations (1)

Maastricht University Medical Centre+; 🇳🇱 Maastricht, Limburg, Netherlands