Using the DNA Exome Sequencing Tools to Explore the Association Among Gene Sequence, TCM Pattern, TCM Tongue Diagnosis and TCM Pulse Diagnosis for Sjögren's Syndrome
试验速览
- 阶段
- 不适用
- 入组人数
- 40
- 试验地点
- 1
- 主要终点
- Immunity-related gene expression
研究概览
简要总结
To explore the association among gene, TCM pattern, TCM tongue diagnosis and TCM pulse diagnosis with the DNA Exome sequencing tools for Sjögren's syndrome
详细描述
Objective: To explore the association among gene, TCM pattern, TCM tongue diagnosis and TCM pulse diagnosis with the DNA Exome sequencing tools for Sjögren's syndrome
Method:
This study wants to establish the reference value of DNA gene variants of healthy control for comparing with the Sjögren's syndrome subjects. And the investigators want to use the DNA Exome sequencing tools, proteomics and oral microbiome to explore the association of gene expression between at least 30 Sjögren's syndrome subjects and at least 10 healthy control subjects. Finally, the investigators also analyze the association among gene expression, proteomics and oral microbiome, TCM pattern, TCM tongue diagnosis, TCM pulse diagnosis, and heart rate variability (HRV) for this purpose.
Expected Results:
- To evaluate the difference among TCM pattern, TCM tongue diagnosis, TCM pulse diagnosis, and HRV for the Sjögren's syndrome and healthy control.
- To evaluate the immunity-related gene expression for the Sjögren's syndrome and healthy control.
- To evaluate the proteomics for the Sjögren's syndrome and healthy control.
- To evaluate the oral microbiome for the Sjögren's syndrome and healthy control.
- To evaluate the association among the immunity-related gene expression, SSA, SSB and RF.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 20 Years 至 75 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •(1) primary or secondary SS
- •(2) aged between 20 and 75 years
- •(3) fulfilled the 2002 American-European Consensus Criteria for SS (AECG)
- •(4) had no abnormal findings of immune, liver, kidney, or blood function evaluations.
排除标准
- •(1)a history of alcohol abuse, diabetes mellitus, or major life-threatening condition
- •(2) pregnancy or breastfeeding
- •(3) steroid pulse therapy within three months prior to the commencement of our study.
- •Health Subjects
- •Inclusion Criteria:
- •(1) aged 20 and 75 years
- •(2) had no chronic inflammatory illness.
- •Exclusion Criteria:
- •(1) a history of alcohol abuse, diabetes mellitus, or major life-threatening condition
- •(2) pregnancy or breastfeeding
- •(3) abnormal findings of immune, liver, kidney, or blood function evaluations
- •(4) total sleeping time insufficiency less than 6 hours before one day of enrollment
- •(5) ever took the conventional medicine or hormone within one month
- •(6) ever encountered the acute illness, allergy reaction, immune, or rheumatic disease within one month.
结局指标
主要结局
Immunity-related gene expression
时间窗: One year
To evaluate the immunity-related gene expression for the Sjögren's syndrome and healthy control. DNA exome combine the GATK (Genome Analysis Toolkit) is a tool for detecting the ratio of immunity-related gene expression.
Heart rate variability (HRV)
时间窗: One year
To evaluate the difference of Heart rate variability (HRV) between Sjögren's syndrome and healthy control. HRV is a tool for detecting the ratio between High-frequency (HF) activity and low-frequency (LF) activity.
Immunity-related microbiome expression
时间窗: One year
To evaluate the oral microbiome for the Sjögren's syndrome and healthy control. Oral microbiome is a tool for detecting the ratio of immunity-related microbiome expression.
TCM tongue diagnosis
时间窗: One year
To evaluate the difference of TCM tongue diagnosis between Sjögren's syndrome and healthy control. TCM tongue diagnosis is a tool for detecting tongue expression.
Immunity-related proteomics expression
时间窗: One year
To evaluate the proteomics for the Sjögren's syndrome and healthy control. Proteomics is a tool for detecting the ratio of immunity-related proteomics expression.
TCM pulse diagnosis
时间窗: One year
To evaluate the difference of TCM pulse diagnosis between Sjögren's syndrome and healthy control. TCM pulse diagnosis is a tool for detecting pulse of radial artery.
TCM pattern
时间窗: One year
To evaluate the difference of TCM pattern between Sjögren's syndrome and healthy control. TCM pattern is a tool for detecting the constitution.
次要结局
未报告次要终点