Diagnosing Invasive Aspergillosis by Polymerase Chain Reaction (PCR) Based Investigation of Bronchoalveolar Lavage Samples During Antifungal Therapy
- Conditions
- Invasive Aspergillosis
- Registration Number
- NCT01448226
- Lead Sponsor
- Heidelberg University
- Brief Summary
Invasive pulmonary aspergillosis (IPA) remains a major cause for morbidity and mortality in patients (pts) with hematologic malignancies. As culture-based methods only yield results in a minority of patients, using non-culture-based methods for detection of aspergillosis in clinical specimens becomes increasingly important. Analyzing bronchoalveolar lavage (BAL) samples with polymerase chain reaction (PCR) is promising, however, the influence of current antifungal drugs on the performance of this diagnostic tool remains controversial.
The aim of the trial is to elucidate on the performance of BAL PCR under antifungal treatment.
- Detailed Description
Invasive pulmonary aspergillosis (IPA) remains a major cause for morbidity and mortality in patients (pts) with hematologic malignancies. As culture-based methods only yield results in a minority of patients, using non-culture-based methods for detection of aspergillosis in clinical specimens becomes increasingly important. Analyzing bronchoalveolar lavage (BAL) samples with polymerase chain reaction (PCR) is promising, however, the influence of current antifungal drugs on the performance of this diagnostic tool remains controversial.
The aim of the trial is to elucidate on the performance of BAL PCR under antifungal treatment.
Patients with high risk of invasive aspergillosis and lung infiltrates are sampled via BAL, the sample is analyzed for fungal DNA by Apsergillus specific PCR. Clinical data including treatment data is assessed and evaluated.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 221
- immunocomprimised patients with high risk of invasive aspergillosis and lung infiltrates
- informed consent
- Children under the age of 5 years
- Informed consent not available
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (22)
Innsbruck University Hospital
🇦🇹Innsbruck, Austria
Bielefeld evangelisches Krankenhaus
🇩🇪Bielefeld, Germany
Bochum University Hospital
🇩🇪Bochum, Germany
Cologne University Hospital
🇩🇪Cologne, Germany
Düsseldorf University Hospital
🇩🇪Düsseldorf, Germany
Erlangen University Hospital
🇩🇪Erlangen, Germany
St.-Antonius Hospital Eschweiler
🇩🇪Eschweiler, Germany
General Hospital Frankfurt (Oder)
🇩🇪Frankfurt (Oder), Germany
Freiburg University Hospital
🇩🇪Freiburg, Germany
Halle University Hospital
🇩🇪Halle (Saale), Germany
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