Two-day Bed Rest, Insulin Sensitivity and Muscle Protein Synthesis

Not Applicable

Withdrawn

• Conditions: Healthy
• Interventions: Behavioral: Habitual activity; Behavioral: Bed rest

• Registration Number: NCT03877822
• Lead Sponsor: University of Exeter

Brief Summary

The impact of two days bed rest versus two days of habitual activity on insulin sensitivity and cumulative muscle protein synthesis will be investigated in healthy young males

Detailed Description

To study the mechanisms underlying inactivity-induced muscle atrophy, the impact of two days bed rest versus two days of habitual activity on insulin sensitivity and cumulative muscle protein synthesis will be investigated in healthy young males

Study Timeline

• Study First Submitted: 2019-02-28
• Study First Submitted QC: 2019-03-14
• Study First Posted: 2019-03-18
• Study Start: 2021-09-01
• Primary Completion: 2021-09-01
• Study Completion: 2021-09-01
• Status Verified: 2021-11
• Last Update Submitted: 2021-11-09
• Last Update Posted: 2021-11-17

Recruitment & Eligibility

• Status: WITHDRAWN
• Sex: Male
• Target Recruitment: Not specified

Inclusion Criteria

• Male
• Aged 18-40

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Exclusion Criteria

• Smoker
• Any diagnosed metabolic impairment (e.g. type 1 or 2 Diabetes)
• Any diagnosed cardiovascular disease (e.g. deep vein thrombosis or hypertension)
• Chronic use of any prescribed or over the counter pharmaceuticals (that may modulate muscle protein metabolism).
• A personal or family history of thrombosis, epilepsy, seizures or schizophrenia.
• Any known disorders in muscle metabolism
• Regular use of nutritional supplements (i.e. ingestion more than twice per week during the last month)
• Metallic implants (including heart pacemaker, cochlear implants, medication pumps, surgical clips, plates or screws).
• Allergy to lidocaine
• Recent (within the last 6 months) or current musculoskeletal injury (e.g. leg fracture)

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Habitual activity and 2 days bed rest	Bed rest	Participants will undergo 2 days of habitual activity and 2 days bed rest
Habitual activity and 2 days bed rest	Habitual activity	Participants will undergo 2 days of habitual activity and 2 days bed rest

Primary Outcome Measures

Name	Time	Method
Change in whole-body insulin sensitivity	During 2 days of habitual physical activity and 2 days of bed rest. Blood glucose and insulin are monitored for 2 hours following ingestion of a standardized meal	Change in whole-body insulin sensitivity via meal tolerance test

Secondary Outcome Measures

Name	Time	Method
Change in cumulative muscle protein synthesis	During 2 days of habitual physical activity and 2 days of bed rest	Cumulative muscle protein synthesis via deuterium oxide ingestion. The incorporation of deuterated alanine in muscle is expressed relative to body water enrichments, to obtain muscle protein synthesis in %/day
Change in continuous glucose	Continuous glucose, during the 2 days of habitual activity and 2 days of bed rest	Change in continuous glucose via continuous glucose monitoring.
Change in muscle volume	Immediately before and immediately after the 2-day bed rest period	Change in muscle volume via MRI