CD160 Ans Corneal Graft: Expression in Corneal Vessels
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- CHU de Reims
- 入组人数
- 43
- 试验地点
- 1
- 主要终点
- Micro-vascular densities
研究概览
简要总结
Despite improvements in corneal transplantation, anti-angiogenic and anti-lymphangiogenic factors remain to be be identified. CD160 is an anti-angiogenic target: its expression seems to be restricted to some immune cells and to activated endothelial cells. We hypothesized that CD160 is expressed in blood and lymphatic human corneal neovessels and is involved in corneal graft rejection pathogenesis
详细描述
After informed consent, patients enrolled for corneal graft surgery at the Robert Debré University Hospital, Reims, France, will be included.
Routine general and eye examination including cornea photographs will be performed.
During surgery, a quarter of patients' cornea and of donors' corneoscleral rim will be spared for immunohistochemistry analyzes.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •18-year-old or more patients
- •enrolled for penetrating keratoplasty or deep anterior lamellar keratoplasty surgeries at the Robert Debré University Hospital, Reims, France
- •considered able to give an informed written consent
排除标准
- 未提供
研究组 & 干预措施
HOSTS
patients ongoing corneal transplantation procedure which a full-thickness cornea from the host is replaced by a graft from a donor
干预措施: Immunohistochemistry and clinical analyses on patients and corneas (Biological)
DONORS
grafts from donors that would be analyzed
干预措施: Immunohistochemistry and clinical analyses on patients and corneas (Biological)
结局指标
主要结局
Micro-vascular densities
时间窗: 1 year
neovascularization scores quantification of blood (anti-ERG+/anti-D2-40-) and lymphatic (anti-ERG+/anti-D2-40+) vascular structures ; Percentages of those vascular structures marked with anti-CD160
次要结局
- Percentages of those vascular structures marked with anti-CD160 and/or CD105 and/or aSMA(1 year)
- Evaluation of clinical blood vascular densities on photographs with help of ImageJ software(1 year)